KnockOut™ DMEM

KnockOut™ D-MEM ist ein Basismedium, das für das Wachstum undifferenzierter embryonaler und induzierter pluripotenter Stammzellen (1) optimiert ist. Die osmotischeWeitere Informationen

Katalognummer	Menge
10829018	500 mL

Katalognummer 10829018

Preis (EUR)

29,19

Each

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Menge:

500 mL

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Preis (EUR)

29,19

Each

Zum Warenkorb hinzufügen

KnockOut™ D-MEM ist ein Basismedium, das für das Wachstum undifferenzierter embryonaler und induzierter pluripotenter Stammzellen (1) optimiert ist. Die osmotische Konzentration ist so optimiert, dass sie in etwa der Osmolarität in embryonalem Mausgewebe entspricht.

Enthält kein L-Glutamin.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

ZelltypStammzellen (embryonal), Stammzellen (iPS – induzierte pluripotente Stammzellen)

Fertigungsqualität, HerstellungsqualitätcGMP für medizinische Geräte, 21 CFR Teil 820 und ISO 13485

ProduktlinieKnockOut™

ProdukttypKnockOut DMEM

Menge500 mL

VersandbedingungRaumtemperatur

FormFlüssig

Mit AdditivenHoher Glukosegehalt, Phenolrot

Ohne AdditiveKein Glutamin

Unit SizeEach

Inhalt und Lagerung

Im Kühlschrank (2–8 °C) aufbewahren und vor Licht schützen.

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Häufig gestellte Fragen (FAQ)

Can KnockOut SR be used to culture mouse ESCs in feeder-free conditions?

Yes, mouse embryonic stem cells can be cultured using KnockOut SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can KnockOut SR be used to culture mouse ESCs on feeder cells?

Yes, KnockOut SR was designed to culture mouse ES cells in undifferentiated conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

What are ES cells?

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.