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Invitrogen™
dNTP-Mix (je 10 mM)
Invitrogen 10 mM dNTP-Mix ist eine Mischung aus vier Nukleotiden (dATP, dCTP, dGTP, dTTP) in 0,6 mM Tris-HCl. Jedes NukleotidWeitere Informationen
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| Katalognummer | Menge |
|---|---|
| 18427013 | 100 μl |
| 18427089 | 5 x 1 ml |
| 18427088 | 1 ml |
Katalognummer 18427013
Preis (EUR)
126,65
Online Exclusive
143,00Ersparnis 16,35 (11%)
Each
Vorrätig
Menge:
100 μl
Preis (EUR)
126,65
Online Exclusive
143,00Ersparnis 16,35 (11%)
Each
Invitrogen 10 mM dNTP-Mix ist eine Mischung aus vier Nukleotiden (dATP, dCTP, dGTP, dTTP) in 0,6 mM Tris-HCl. Jedes Nukleotid hat eine Konzentration von 10 mM. Der 10 mM dNTP-Mix eignet sich für Polymerase-Kettenreaktion (PCR), Sequenzierung, Fill-in-Reaktionen, Nick-Translation, cDNA-Synthese und TdT-Tailing-Reaktionen.
Die Invitrogen dNTP-Herstellung erfüllt die höchsten Industriestandards:
• Hergestellt nach ISO 9001
• Hergestellt in Reinräumen der Klasse D
Merkmale:
• Chemisch synthetisiert
• pH 7,5
• >99 % Reinheit, bestätigt durch HPLC
• Bei –20 °C 2 Jahre stabil
• Frei von Inhibitoren von qPCR, PCR, reverse Transkription
• Frei von DNasen und RNasen
• Frei von humaner und E. coli-DNA
Anwendungen:
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA-Synthese
• High-Fidelity- und Long-Range-PCR
• Isotherme Amplifikation
• DNA-Markierung
• Klonierung
• Sanger und Next-Generation-Sequenzierung (NGS)
Nur für Forschungszwecke.Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Konzentration40 mmol
Menge100 μl
VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis
Zur Verwendung mit (Anwendung)Standard-PCR, RT-PCR, Real-Time PCR (qPCR)
FormFlüssig
Marker oder FarbstoffUnmarkiert
Unit SizeEach
Inhalt und Lagerung
µ1 x 100 l-Fläschchen
Die Mischung enthält alle vier dNTPs, jeweils mit einer Endkonzentration von 10 mM in Tris-HCl (pH 7,5). Bei -20 °C lagern.
Die Mischung enthält alle vier dNTPs, jeweils mit einer Endkonzentration von 10 mM in Tris-HCl (pH 7,5). Bei -20 °C lagern.
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Zertifikate
Nach Chargennummer oder Teil-Chargennummer suchen
Chargen-Nr.Certificate TypeDateCatalog Number(s)
3146024Certificate of Analysis30. Juni 202518427088
3037356Certificate of Analysis05. Mai 202518427088
2963963Certificate of Analysis18. Feb. 202518427088
2994125Certificate of Analysis16. Sept. 202418427013
2943386Certificate of Analysis30. Aug. 202418427088
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Sicherheitsdatenblätter
SDBHäufig gestellte Fragen (FAQ)
The following components are available as stand-alone items:
- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)
Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions
Uracil-N-glycosylase (UNG) has activity in the temperature range of 4 degrees C to 55 degrees C, and is not completely inactivated by incubation at 95 degrees C. It is recommended that the annealing temperature be at or above 55 degrees C when using this enzyme with any of our PCR or Sequence Detection Systems kits to avoid possible degradation of newly synthesized dU-containing PCR products by residual UNG activity. For this reason, it is also recommended that the post-PCR soak be maintained at 72 degrees C (instead of 4 degrees C) if analysis of the PCR product will be performed using a gel-based method.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
The control primers generate a 300 and a 500 bp amplicon from the lambda control DNA. The Lambda Multiplex Control primers, as well as the Lambda Control DNA are provided in this kit.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Zitierungen und Referenzen (1)
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Zitierungen und Referenzen
Abstract
Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12119387
Much of the information available about factors that affect mRNA decay in Escherichia coli, and by inference in other bacteria, has been gleaned from study of less than 25 of the approximately 4,300 predicted E. coli messages. To investigate these factors more broadly, we examined the half-lives and steady-state abundance
1 total citations