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Katalognummer | Menge | Produkttyp |
---|---|---|
A72126 | 2000 μL | Reaktionspuffer |
A72123 | 1600 μg | Lyo-ready T4 Gene 32 Protein |
A72124 | 120 μg | Lyo-ready T4 UvsX Protein |
A72125 | 120 μg | Lyo-ready T4 UvsY Protein |
A72127 | 100 Reactions | Lyo-ready RPA Kit |
A72128 | 500 Reactions | Lyo-ready RPA Kit |
Features of Lyo-ready RPA products
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Try Lyo-Ready Bst DNA Polymerase for rapid and sensitive detection of various pathogens.
Simple, sensitive, and specific DNA amplification using RPA technology.
PCR primers can be used with the Lyo-ready RPA Kit if they are at least 30 nt long. RPA primers can be designed using Primer3.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
All RPA reagents (including Lyo-ready T4 UvsX Protein (Cat. No. A72124), Lyo-ready T4 UvsY Protein (Cat. No. A72125), Lyo-ready T4 Gene 32 Protein (Cat. No. A72123), Lyo-ready Bst DNA Polymerase (Cat. No. A56655), and RPA Reaction Buffer (2X) (Cat. No. A72126)) are lyo-ready, ensuring that enzymes and buffer formulations are glycerol-free. However, the formulations do not contain excipients needed for the lyophilization process.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Lyo-ready RPA Kit and stand-alone reagents come in a storage buffer that does not contain glycerol. Product freezing doesn't affect its performance or stability. We do not recommend freeze-thawing Lyo-ready RPA Kit and stand-alone reagents for more than 20 cycles.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
Lyo-ready RPA Kit can amplify DNA in temperatures ranging from 37 to 45 degrees C. To ensure the best performance, we recommend using the optimal reaction temperature of 42 degrees C.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
We recommend using SuperScript IV Reverse Transcriptase (Cat. No. 18090010, 18090050, 18090200) for the best results in RT-RPA. Guidelines for use of SuperScript IV Reverse Transcriptase in RT-RPA application can be found in the Lyo-ready RPA Kit user guide. Other reverse transcriptases such as Maxima H Minus Reverse Transcriptase (Cat. No. EP0751) have also been tested and can be used in RT-RPA application. The list of Lyo-ready reverse transcriptases can be found here: thermofisher.com/lyo-ready.
Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.
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