Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen™

Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
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KatalognummerMengeProdukttyp
A721262000 μLReaktionspuffer
A721231600 μgLyo-ready T4 Gene 32 Protein
A72124120 μgLyo-ready T4 UvsX Protein
A72125120 μgLyo-ready T4 UvsY Protein
A72127100 ReactionsLyo-ready RPA Kit
A72128500 ReactionsLyo-ready RPA Kit
Katalognummer A72126
Preis (EUR)
80,25
Each
-
Zum Warenkorb hinzufügen
Menge:
2000 μL
Produkttyp:
Reaktionspuffer
Preis (EUR)
80,25
Each
Zum Warenkorb hinzufügen
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Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications
Konzentration2X
Optimale Reaktionstemperatur42 °C
ReaktionszeitUp to 20 min.
VersandbedingungDry Ice
Zur Verwendung mit (Anwendung)RPA, RT-RPA
FormFlüssig
ProdukttypReaktionspuffer
Menge2000 μL
ForschungskategorieNucleic Acid Amplification
Unit SizeEach
Inhalt und Lagerung
2X RPA Reaction Buffer, 2 mL, Store at -25°C to -15°C.
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Zertifikate

Chargen-Nr.Certificate TypeDateCatalog Number(s)
3271828Certificate of Analysis01. Juli 2025A72124
3271840Certificate of Analysis01. Juli 2025A72124
3269016Certificate of Analysis26. Juni 2025A72124
3265634Certificate of Analysis20. Juni 2025A72125
3266139Certificate of Analysis20. Juni 2025A72124
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Sicherheitsdatenblätter

Häufig gestellte Fragen (FAQ)

PCR primers can be used with the Lyo-ready RPA Kit if they are at least 30 nt long. RPA primers can be designed using Primer3.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

All RPA reagents (including Lyo-ready T4 UvsX Protein (Cat. No. A72124), Lyo-ready T4 UvsY Protein (Cat. No. A72125), Lyo-ready T4 Gene 32 Protein (Cat. No. A72123), Lyo-ready Bst DNA Polymerase (Cat. No. A56655), and RPA Reaction Buffer (2X) (Cat. No. A72126)) are lyo-ready, ensuring that enzymes and buffer formulations are glycerol-free. However, the formulations do not contain excipients needed for the lyophilization process.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Lyo-ready RPA Kit and stand-alone reagents come in a storage buffer that does not contain glycerol. Product freezing doesn't affect its performance or stability. We do not recommend freeze-thawing Lyo-ready RPA Kit and stand-alone reagents for more than 20 cycles.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Lyo-ready RPA Kit can amplify DNA in temperatures ranging from 37 to 45 degrees C. To ensure the best performance, we recommend using the optimal reaction temperature of 42 degrees C.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

We recommend using SuperScript IV Reverse Transcriptase (Cat. No. 18090010, 18090050, 18090200) for the best results in RT-RPA. Guidelines for use of SuperScript IV Reverse Transcriptase in RT-RPA application can be found in the Lyo-ready RPA Kit user guide. Other reverse transcriptases such as Maxima H Minus Reverse Transcriptase (Cat. No. EP0751) have also been tested and can be used in RT-RPA application. The list of Lyo-ready reverse transcriptases can be found here: thermofisher.com/lyo-ready.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

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