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RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES
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Gibco™

RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aRead more
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Catalog NumberQuantityWith AdditivesWithout Additives
72400013
also known as 72400-013
100 mLGlutaMAX, HEPES, Phenol RedNo Sodium Pyruvate
72400021
also known as 72400-021
500 mLGlutaMAX, HEPES, Phenol Red, Sodium BicarbonateNo Sodium Pyruvate
72400054
also known as 72400-054
10 x 500 mLGlutaMAX, HEPES, Phenol Red, Sodium BicarbonateNo Sodium Pyruvate
72400112
also known as 72400-112
20 x 100 mLGlutaMAX, HEPES, Phenol RedNo Sodium Pyruvate
Catalog number 72400013
also known as 72400-013
Price (EUR)
21,05
Each
Estimated availability date 21-Jul-2025
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Quantity:
100 mL
With Additives:
GlutaMAX, HEPES, Phenol Red
Without Additives:
No Sodium Pyruvate
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Price (EUR)
21,05
Each
Add to cart
Ask our AI about this Product
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
With
• GlutaMax™ I
• Phenol Red
• HEPES

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. Gibco™ RPMI 1640 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Dual-Site cGMP Manufacturing
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ RPMI 1640 product made in our Grand Island facility (72400-047). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1 X
FormLiquid
Product TypeRPMI 1640
Serum LevelStandard Serum Supplementation
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesGlutaMAX, HEPES, Phenol Red
Without AdditivesNo Sodium Pyruvate
Product LineGibco™, GlutaMAX™
Quantity100 mL
Shipping ConditionRoom Temperature
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3141660Certificate of AnalysisJun 27, 202572400047, 72400146, 72400013, 72400021, 72400153
3221713Certificate of AnalysisJun 14, 202572400047, 72400146, 72400013, 72400021, 72400153
3209607Certificate of AnalysisJun 13, 202572400047, 72400146, 72400013, 72400021, 72400153
3126851Certificate of AnalysisJun 05, 202572400047, 72400146, 72400013, 72400021, 72400153
3224718Certificate of AnalysisMay 31, 202572400047, 72400146, 72400013, 72400021, 72400153
5 results displayed, search above for a specific certificate

Safety Data Sheets

Frequently asked questions (FAQs)

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

In all media containing GlutaMAX supplement dipeptides as a substitute for L-glutamine, concentration is equimolar with the L-glutamine in the original formulation.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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