Pierce™ Silver Stain Kit
Pierce™ Silver Stain Kit
Thermo Scientific™

Pierce™ Silver Stain Kit

The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels,Read more
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Catalog NumberQuantity
246121 L
24612X33 x 1 L
Catalog number 24612
Price (EUR)
188,65
온라인 행사
228,00
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1 L
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Price (EUR)
188,65
온라인 행사
228,00
Save 39,35 (17%)
Each
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The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Compare all silver stains ›

Features:
Sensitivity—detect proteins at 0.25 ng
Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Product LinePierce™
Product TypeStain Kit
Quantity1 L
Target MoleculeProtein
Label or DyeSilver
Unit SizeEach
Contents & Storage
Sufficient For: 20 mini gels (SDS-PAGE)
• Silver Stain Sensitizer, 2 mL
• Silver Stain, 500 mL
• Silver Stain Enhancer, 25 mL
• Silver Stain Developer, 500 mL

Store at room temperature.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3213979ACertificate of AnalysisJul 03, 202524612
3213979Certificate of AnalysisJul 03, 202524612
3212115ACertificate of AnalysisJun 03, 202524612
3212115Certificate of AnalysisJun 03, 202524612
AC406093Certificate of AnalysisApr 30, 202524612
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Safety Data Sheets

Frequently asked questions (FAQs)

Yes. References in the literature and molecular biology protocols indicate that nucleic acids can be detected in polyacrylamide gels with silver stains of this type.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Yes. See Tech Tip: Process Stained Polyacrylamide Gels for Mass Spectrometry (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0050-Stained-gels-for-MS.pdf). Alternatively, use our Thermo Scientific Silver Stain for MS (Cat No. 24600), which is the same silver stain kit but also includes destaining reagents and a protocol optimized for maximum protein recovery.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

SDS, glycine, amines and phosphates interfere with the staining method; this is why initial wash and fixing steps are necessary. Silver staining is also sensitive to pH conditions, chelators (sequester silver), and strong oxidants or reductants. To avoid these contaminants, use ultrapure water and clean equipment (avoid metal utensils), and avoid touching the gel except at the edges. Prepare working solutions of stain, sensitizer and developer immediately before use.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The 1 minute sensitization and 2 x 1 minute water wash steps are important for optimum stain performance. Development time (2-3 minutes) is also critical, and stop solution must be added as soon as desired development occurs.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The gel is washed and fixed to remove electrophoresis buffer salts. The silver stain is added, releasing silver ions that interact with the proteins. The developer is then added causing the protein bands to darken as the bound silver reduces. The color development is stopped by lowering the pH with acetic acid.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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