Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Quantity |
---|---|
24612 | 1 L |
24612X3 | 3 x 1 L |
Featuring higher throughput and built-in cooling for consistent protein transfer
Learn more ›
Yes. References in the literature and molecular biology protocols indicate that nucleic acids can be detected in polyacrylamide gels with silver stains of this type.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes. See Tech Tip: Process Stained Polyacrylamide Gels for Mass Spectrometry (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0050-Stained-gels-for-MS.pdf). Alternatively, use our Thermo Scientific Silver Stain for MS (Cat No. 24600), which is the same silver stain kit but also includes destaining reagents and a protocol optimized for maximum protein recovery.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
SDS, glycine, amines and phosphates interfere with the staining method; this is why initial wash and fixing steps are necessary. Silver staining is also sensitive to pH conditions, chelators (sequester silver), and strong oxidants or reductants. To avoid these contaminants, use ultrapure water and clean equipment (avoid metal utensils), and avoid touching the gel except at the edges. Prepare working solutions of stain, sensitizer and developer immediately before use.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The 1 minute sensitization and 2 x 1 minute water wash steps are important for optimum stain performance. Development time (2-3 minutes) is also critical, and stop solution must be added as soon as desired development occurs.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The gel is washed and fixed to remove electrophoresis buffer salts. The silver stain is added, releasing silver ions that interact with the proteins. The developer is then added causing the protein bands to darken as the bound silver reduces. The color development is stopped by lowering the pH with acetic acid.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Share catalog number, name or link