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StemPro™ Adipogenesis Differentiation Kit
StemPro™ Adipogenesis Differentiation Kit
Gibco™

StemPro™ Adipogenesis Differentiation Kit

ヒト間葉系幹細胞から脂肪細胞への完全な分化に対応StemPro™脂質生成分化キットは、間葉系幹細胞(MSC)を組織培養容器内で脂肪生成分化する目的で開発されました。このキットには、脂肪生成経路にMSCを誘導し、脂肪細胞を生成するために必要なすべての試薬が含まれています。StemPro™脂質生成分化キットをStemPro™ MSC SFMまたはMesenPRO詳細を見る
製品番号(カタログ番号)数量
A10070011 Kit
製品番号(カタログ番号) A1007001
価格(JPY)
49,300
Each
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数量:
1 Kit
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ヒト間葉系幹細胞から脂肪細胞への完全な分化に対応

StemPro™脂質生成分化キットは、間葉系幹細胞(MSC)を組織培養容器内で脂肪生成分化する目的で開発されました。このキットには、脂肪生成経路にMSCを誘導し、脂肪細胞を生成するために必要なすべての試薬が含まれています。StemPro™脂質生成分化キットをStemPro™ MSC SFMまたはMesenPRO RS™培地と組み合わせて使用することで、MSCの単離、増殖、脂質小胞形成脂肪細胞への分化のための標準化された培養ワークフローソリューションが実現します。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞タイプ幹細胞(間葉系)
培養環境CO2
エンドトキシンレベル≦50 EU/mL、≦50 EU/ml
フォーマット液体
グルコース高グルコース
グルタミンGlutaMAX™-I
HEPESバッファーHEPESなし
無機塩類カルシウム、リン酸ナトリウム
フェノールレッド指示薬フェノールレッドなし
純度または品質グレード研究グレード
数量1 Kit
研究カテゴリー分化
血清レベル標準血清
炭酸水素ナトリウムバッファー重炭酸ナトリウム
ピルビン酸ナトリウム添加剤ピルビン酸ナトリウム
ヒト
サプリメント脂肪生成サプリメント
使用対象(アプリケーション)幹細胞の分化
製品ラインGibco™、StemPro™
製品タイプAdipogenesis Differentiation Kit
Unit SizeEach
組成および保存条件
このキットは、別々の保存要件を持つ2つの部品にして出荷します。これらのコンポーネントは個別には販売されていません。
•StemPro™脂肪細胞分化用基礎培地(100 ml、2℃~8℃の暗所で保存)
• StemPro™脂肪生成サプリメント(10 ml、-5℃~-20℃の暗所で保存)

基礎培地は室温で出荷され、サプリメントはドライアイスで出荷されます。
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What are mesenchymal stem cells?
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よくあるご質問(FAQ)

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).

引用および参考文献 (3)

引用および参考文献
Abstract
Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.
Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,
Journal:
PubMed ID:26564250
'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More
Alginate-Encapsulation for the Improved Hypothermic Preservation of Human Adipose-Derived Stem Cells.
Authors:Swioklo S, Constantinescu A, Connon CJ,
Journal:Stem Cells Transl Med
PubMed ID:26826163
: Despite considerable progress within the cell therapy industry, unmet bioprocessing and logistical challenges associated with the storage and distribution of cells between sites of manufacture and the clinic exist. We examined whether hypothermic (4°C-23°C) preservation of human adipose-derived stem cells could be improved through their encapsulation in 1.2% calcium ... More
Human Synovial MSC Good Manufacturing Practices for Articular Cartilage Regeneration.
Authors:Fernandes TL, Kimura HA, Pinheiro CCG, Shimomura K, Nakamura N, Ferreira JRM, Gomoll AH, Hernandez AJ, Bueno DF,
Journal:Tissue Eng Part C Methods
PubMed ID:30412046
cartilage restoration is a desperately needed bridge for patients with symptomatic cartilage lesions. Chondral lesion is a pathology with high prevalence, reaching as much as 63% of general population and 36% among athletes. Despite Autologous Chondrocyte Implantation (ACI) versatility, it still fails to fully reproduce hyaline articular cartilage characteristics. Mesenchymal ... More
3 total citations

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