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인용 및 참조 문헌 (3)
Gibco™
Cell Dissociation Buffer, enzyme-free, PBS
Gibco™ Cell Dissociation Buffer는 무칼슘, 무마그네슘 인산염완충식염수(PBS)에 염, 킬레이트제, 세포조절제가 들어있는 막여과된 등장성 무효소 용액입니다. Gibco™ Cell Dissociation Buffer는 리간드자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| 13151014 | 100 mL |
카탈로그 번호 13151014
제품 가격(KRW)
81,000
Each
-
수량:
100 mL
제품 가격(KRW)
81,000
Each
Gibco™ Cell Dissociation Buffer는 무칼슘, 무마그네슘 인산염완충식염수(PBS)에 염, 킬레이트제, 세포조절제가 들어있는 막여과된 등장성 무효소 용액입니다. Gibco™ Cell Dissociation Buffer는 리간드 결합, 유세포분석, 면역조직화학 등 세포 표면이 온전한 단백질이 필요한 연구에서 포유류 세포를 무리없이 해리하는데 적합합니다. Gibco™ Cell Dissociation Buffer는 HeLa 및 NIH 3T3 등 약하게 붙어있는 세포주에 적합하며 강하게 부착된 세포의 일반적 계대배양에는 권장되지 않습니다. 해리 프로토콜을 본사 Technical Reference Library에서 이용할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주HeLa, NIH 3T3
킬레이터EDTA
수량100 mL
비고Room Temperature Stable
유통 기한24 Months
배송 조건Room Temperature
분류Animal Origin-free
제품 유형Cell Culture Dissociation Reagent
멸균Sterile-filtered
첨가제 포함EDTA
Unit SizeEach
구성 및 보관
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
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Certificates | 증명서
Lot 번호 또는 부분 Lot 번호로 검색하십시오.
Lot 번호Certificate TypeDateCatalog Number(s)
3146837Certificate of Analysis2025년 6월 12일13151014
2572391Certificate of Analysis2025년 1월 29일13151014
2572392Certificate of Analysis2025년 1월 29일13151014
2944208Certificate of Analysis2025년 1월 25일13151014
A0446664Certificate of Analysis2024년 5월 09일131510250, 131510010, 131510025, 13151014
시험 성적서 관련 5 개의 결과가 검색되었습니다.
Safety Data Sheets | 물질 안전 보건 자료
SDS자주 묻는 질문(FAQ)
Below are QC test specifications for pH and osmolality for this product:
- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
인용 및 참조 문헌 (3)
Search citations by name, author, journal title or abstract text
인용 및 참조 문헌
Abstract
Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.
Journal:Nat Protoc
PubMed ID:21372811
'In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin(-)CD34(+)CD43(+)CD45(+) multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9
Long term non-invasive imaging of embryonic stem cells using reporter genes.
Journal:Nat Protoc
PubMed ID:19617890
'Development of non-invasive and accurate methods to track cell fate after delivery will greatly expedite transition of embryonic stem (ES) cell therapy to the clinic. In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines
Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12361980
Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epidemiological studies have suggested
3 total citations