Mesophilic and thermophilic DNA polymerases for different polymerization reactions, DNA end-blunting and amplification, labeling and others
Properties of DNA polymerases
|
| Polymerase | Applications | Template | 3' - 5' exo- nuclease | 5' - 3' exo- nuclease | Strand displace- ment |
|---|
| DNA Polymerase I | - DNA labeling by nick-translation
- Second strand cDNA synthesis
| DNA |  |  | |
| Klenow Fragment | - DNA blunting by fill-in 5'- overhangs
- Random-primed DNA labeling
- Labeling by fill-in 5'- overhangs of dsDNA
- DNA sequencing
- Site-specific mutagenesis
- Second strand cDNA synthesis
| DNA | | | |
| Klenow Fragment, exo- | - Random-primed DNA labeling
- Labeling by fill-in 5'- overhangs of dsDNA
- Strand displacement amplification
- DNA sequencing
| DNA | | | |
| T4 DNA Polymerase | - Blunting of DNA with 5'- or 3'- overhangs
- Blunting of PCR products with 3'-dA overhangs
- DNA labeling
- Site-directed mutagenesis
- Ligation-independent cloning
| DNA |  | | |
| T7 DNA Polymerase | - Purification of covalently closed circular DNA
- Primer extension reactions on long templates
- DNA 3'-end labeling
- Strand extensions in site directed mutagenesis
- Fill-in blunting of 5'-overhang DNA
- Second strand cDNA synthesis
- In situ detection of DNA fragmentation associated with apoptosis
| DNA | | | |
| Terminal deoxynucleotidyl transferase (TdT) | - Production of synthetic homo-, heteropolymers
- Homo polymeric tailing of linear duplex DNA with 3'-OH terminus
- Oligodeoxyribonucleotide and DNA labeling
- 5'-RACE
| ssDNA
dsDNA
ssRNA
dsRNA
| | | NA |
| Terminal deoxynucleotidyl transferase (TdT), Recombinant | - Production of synthetic homo-, heteropolymers
- Homo polymeric tailing of linear duplex DNA with 3'-OH terminus
- Oligodeoxyribonucleotide and DNA labeling
- PCR, RNA analysis, genomic DNA cloning, 5' RACE, sequencing, and labeling of DNA probes
| ssDNA
dsDNA
ssRNA
dsRNA
| | | NA |
| phi29 DNA Polymerase | - Rolling circle amplification
- Multiple displacement amplification
- Whole genome amplification(WGA)
- DNA sequencing
- Protein-primed DNA amplification
- RNA-primed DNA amplification
| DNA | | | |
| Bsm DNA Polymerase | - Loop-mediated isothermal amplification (LAMP)
- Whole genome amplification (WGA)
- Ramification amplification (RAM)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
| DNA | | | |
EP0041,EP0042,EP0051,EP0052,EP0054,EP0061,EP0062,EP0081,EP0091,EP0092,EP0094,EP0161,EP0162,72033.2500.UN,EP0421,EP0422,EP0691
Ordering information
EP0421,EP0422,EP0041,EP0052,EP0162,EP0042,EP0691,EP0054,EP0081,EP0092,EP0061,EP0094,EP0051,EP0062,EP0161,EP0091