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Precast Agarose Gels |
Fast, convenient DNA electrophoresis
E-Gel precast gels are bufferless agarose gels with electrodes embedded in the agarose matrix. Each precast agarose gel contains an ion generating system, a pH balancing system, and DNA stain packaged inside a transparent plastic cassette. It also contains two ion exchange matrices (IEMs) that are in contact with the gel and electrodes. The IEMs supply a continuous flow of ions throughout the precast gel resulting in a sustained electric field required for running the gel.
Features of E-Gel precast agarose gels
- Ready-to-use—no gel preparation or additional buffers required
- Safer analysis—choose E-Gels containing SYBR Safe DNA stain, a safer alternative to ethidium bromide
- High performance—detect 2–3 ng of sample DNA in less than 30 minutes
- Wide variety of applications—large selection of agarose percentages, well formats, and throughput capacities to suit your applications
Types of precast agarose gels
The E-gel precast agarose gels are available in multiple formats to suit your applications.
E-Gel SizeSelect II gels are double-comb, precast agarose gels designed for separation and recovery of DNA fragments.
Advantages of E-Gel SizeSelect II gels:
- Optimized for tight, consistent recovery of DNA fragments <1 Kb in size
- Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
- Use recovered DNA directly in cloning reactions
E-Gel CloneWell II gels are double-comb, precast agarose gels designed for separation and retrieval of DNA fragments of choice.
Advantages of E-Gel CloneWell II gels:
- Gel-purify your DNA in 3 simple steps (Figure 1)
- Designed to obtain improved cloning efficiencies
- View bands in real time and help minimize DNA damage
- Collect multiple DNA bands from the same gel lane
Figure 1. Gel purification in three steps.
Improved cloning efficiencies
Exposure of your DNA sample to UV light during visualization may lead to DNA damage and reduced cloning efficiencies. Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap System helps eliminate UV damage and can improve cloning efficiency compared to conventional methods. Results obtained using the TOPO TA Cloning Kit after E-Gel CloneWell II gel purification, compared to a conventional method, are shown in Figure 2.
Click image to enlarge
Figure 2. Improved cloning efficiency using E-Gel CloneWell II agarose gels with blue-light transillumination. A PCR reaction containing an 850 bp amplicon was separated on either an E-Gel CloneWell agarose gel or a traditional agarose gel containing ethidium bromide. The DNA band retrieved from the E-Gel CloneWell agarose gel was visualized using blue-light transillumination. DNA separated on the traditional gel was viewed using UV light and isolated by first cutting a gel slice with a razor blade and then using a commercially available gel extraction kit. In both cases, the exposure of the DNA to the light source was 15, 30, or 60 sec. Both fragment samples were cloned using the TOPO TA Cloning Kit and transformed into Invitrogen MultiShot TOP10 chemically competent cells. Shown are average numbers of colony-forming units (CFU) obtained for each exposure time and cloning method.
E-Gel EX gels pre-stained with SYBR Gold DNA stain were developed for exceptional sensitivity and demonstrate up to 5-fold greater sensitivity than comparable gels containing ethidium bromide. These precast gels are available in various agarose concentration which allow resolution of DNA fragments from 50 bp to 5,000 bp. E-Gel EX gels are available in single and double comb formats.
ExploreE-Gel EX Gels
E-Gel NGS gels with SYBR Safe DNA were designed for high range library separation. They come in 0.8% agarose concentration with 11-wells, which allow resolution from 800 bp–10 kb.
E-Gel SizeSelect II gels are double-comb, precast agarose gels designed for separation and recovery of DNA fragments.
Advantages of E-Gel SizeSelect II gels:
- Optimized for tight, consistent recovery of DNA fragments <1 Kb in size
- Purified DNA is compatible with library construction protocols of major generation 2 sequencing platforms
- Use recovered DNA directly in cloning reactions
E-Gel CloneWell II gels are double-comb, precast agarose gels designed for separation and retrieval of DNA fragments of choice.
Advantages of E-Gel CloneWell II gels:
- Gel-purify your DNA in 3 simple steps (Figure 1)
- Designed to obtain improved cloning efficiencies
- View bands in real time and help minimize DNA damage
- Collect multiple DNA bands from the same gel lane
Figure 1. Gel purification in three steps.
Improved cloning efficiencies
Exposure of your DNA sample to UV light during visualization may lead to DNA damage and reduced cloning efficiencies. Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap System helps eliminate UV damage and can improve cloning efficiency compared to conventional methods. Results obtained using the TOPO TA Cloning Kit after E-Gel CloneWell II gel purification, compared to a conventional method, are shown in Figure 2.
Click image to enlarge
Figure 2. Improved cloning efficiency using E-Gel CloneWell II agarose gels with blue-light transillumination. A PCR reaction containing an 850 bp amplicon was separated on either an E-Gel CloneWell agarose gel or a traditional agarose gel containing ethidium bromide. The DNA band retrieved from the E-Gel CloneWell agarose gel was visualized using blue-light transillumination. DNA separated on the traditional gel was viewed using UV light and isolated by first cutting a gel slice with a razor blade and then using a commercially available gel extraction kit. In both cases, the exposure of the DNA to the light source was 15, 30, or 60 sec. Both fragment samples were cloned using the TOPO TA Cloning Kit and transformed into Invitrogen MultiShot TOP10 chemically competent cells. Shown are average numbers of colony-forming units (CFU) obtained for each exposure time and cloning method.
E-Gel EX gels pre-stained with SYBR Gold DNA stain were developed for exceptional sensitivity and demonstrate up to 5-fold greater sensitivity than comparable gels containing ethidium bromide. These precast gels are available in various agarose concentration which allow resolution of DNA fragments from 50 bp to 5,000 bp. E-Gel EX gels are available in single and double comb formats.
ExploreE-Gel EX Gels
E-Gel NGS gels with SYBR Safe DNA were designed for high range library separation. They come in 0.8% agarose concentration with 11-wells, which allow resolution from 800 bp–10 kb.
E-Gel precast agarose gel selection guide
E-Gel precast agarose gels are available in a variety of agarose percentages, well formats, and throughput capacities to suit many applications. Use the guide provided below to choose which precast agarose gel works best for the application in consideration.
E-Gel precast gels for low throughput DNA electrophoresis
For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.
| Goal | General purpose | High sensitivity and faster separation | ||
|---|---|---|---|---|
| Precast gel types | E-Gel | E-Gel EX | ||
| Stain | SYBR Safe DNA stain | SYBR Gold II DNA stain | ||
| Run time | 13–40 min | 5–20 min | ||
| Number of wells | Single row of 11 wells | 22 wells in a double comb design | Single row of 11 wells | 22 wells in a double comb design |
| Agarose % | ||||
| Resolution | 10 bp–5 kb | 50 bp–5 kb | 10 bp–5 kb | 50 bp–5 kb |
| Sample volume | 20 µL | 20 µL | 20 µL | 20 µL |
E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis
E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.
| Goal | High throughput | |
|---|---|---|
| Precast gel type | E-Gel 48-well gels | E-Gel 96-well gels |
| Number of wells | 48 sample wells and 4 marker wells | 96 sample wells and 8 marker wells |
| Stain | SYBR Safe DNA stain or Ethidium bromide | SYBR Safe DNA stain or Ethidium bromide |
| Agarose % | ||
| Resolution | 10 bp–10 kb | 100 bp–10 kb |
| Run time | 17–25 min | 12–17 min |
| Sample volume | 15 µL | 20 µL |
E-Gel precast gels for cloning
Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.
| Goal | High efficiency cloning |
|---|---|
| Precast gel type | E-Gel CloneWell II |
| Number of wells | Two rows of 7 wells (for 7 samples) |
| Stain | SYBR Safe DNA stain |
| Agarose % | 0.8% |
| Resolution | 100 bp–6 kb |
| Run time | 13–28 min |
| Sample volume | 20–25 µL |
E-Gel precast gels for NGS library preparation
For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.
| Goals | Precise DNA fragment library size selection | High range fragment library separation |
|---|---|---|
| Precast gel types | E-Gel SizeSelect II | E-Gel NGS |
| Number of wells | Two rows of 7 wells (for 7 samples) | Single row of 11 wells |
| Stain | SYBR Gold stain | SYBR Safe DNA stain |
| Agarose % | 2.0% | 0.8% |
| Resolution | 50 bp–2 kb | 800 bp–10 kb |
| Run time | 8–20 min | 26–32 min |
| Sample volume | 20–25 µL | 20 µL |
E-Gel precast gels for low throughput DNA electrophoresis
For general purpose DNA electrophoresis or high sensitivity and fast separation; there is an E-Gel precast gel suited for many low throughput agarose gel electrophoresis applications.
| Goal | General purpose | High sensitivity and faster separation | ||
|---|---|---|---|---|
| Precast gel types | E-Gel | E-Gel EX | ||
| Stain | SYBR Safe DNA stain | SYBR Gold II DNA stain | ||
| Run time | 13–40 min | 5–20 min | ||
| Number of wells | Single row of 11 wells | 22 wells in a double comb design | Single row of 11 wells | 22 wells in a double comb design |
| Agarose % | ||||
| Resolution | 10 bp–5 kb | 50 bp–5 kb | 10 bp–5 kb | 50 bp–5 kb |
| Sample volume | 20 µL | 20 µL | 20 µL | 20 µL |
E-Gel 48- and 96-well precast agarose gels for high-throughput DNA electrophoresis
E-Gel 48- and 96-well agarose gels are ideal for high-throughput electrophoresis and analysis of PCR products, plasmid preparations, and restriction digests. E-Gel 48 gels include two rows each containing 24 samples and two molecular weight marker wells with a 3.2 cm length. E-Gel 96 gels contain 96 sample lanes and eight marker lanes, staggered to provide a 1.6 cm run length with resolution between 100 bp and 10 kb. This staggered-well format is compatible with most robotic loading systems.
| Goal | High throughput | |
|---|---|---|
| Precast gel type | E-Gel 48-well gels | E-Gel 96-well gels |
| Number of wells | 48 sample wells and 4 marker wells | 96 sample wells and 8 marker wells |
| Stain | SYBR Safe DNA stain or Ethidium bromide | SYBR Safe DNA stain or Ethidium bromide |
| Agarose % | ||
| Resolution | 10 bp–10 kb | 100 bp–10 kb |
| Run time | 17–25 min | 12–17 min |
| Sample volume | 15 µL | 20 µL |
E-Gel precast gels for cloning
Using E-Gel CloneWell II agarose gels with the E-Gel Power Snap Electrophoresis System eliminates UV damage and improves cloning efficiency. E-Gel CloneWell II agarose gels are double-comb gels designed for the sample to be loaded into the top well, electrophoresed, and bands can then be retrieved once they migrate into the collection well. No additional purification is required.
| Goal | High efficiency cloning |
|---|---|
| Precast gel type | E-Gel CloneWell II |
| Number of wells | Two rows of 7 wells (for 7 samples) |
| Stain | SYBR Safe DNA stain |
| Agarose % | 0.8% |
| Resolution | 100 bp–6 kb |
| Run time | 13–28 min |
| Sample volume | 20–25 µL |
E-Gel precast gels for NGS library preparation
For NGS library prep, the ability to recover a purified DNA fragment is critically important. Choose the E-Gel precast agarose gel that suits the throughput and precision needs of your NGS sample prep workflow.
| Goals | Precise DNA fragment library size selection | High range fragment library separation |
|---|---|---|
| Precast gel types | E-Gel SizeSelect II | E-Gel NGS |
| Number of wells | Two rows of 7 wells (for 7 samples) | Single row of 11 wells |
| Stain | SYBR Gold stain | SYBR Safe DNA stain |
| Agarose % | 2.0% | 0.8% |
| Resolution | 50 bp–2 kb | 800 bp–10 kb |
| Run time | 8–20 min | 26–32 min |
| Sample volume | 20–25 µL | 20 µL |
The E-Gel SizeSelect gels allow users to separate and recover DNA for short read fragment library construction in as little as 15 minutes. That's up to 10 times faster than using conventional gels, and purification kits. E-Gel SizeSelect gels are offered in a 2% agarose concentration and validated for the recovery of fragments in the 150–200 bp size range. Once the DNA is recovered, no additional purification step is required before proceeding to the next step in library construction. E-Gel SizeSelect system has been used successfully in these generation 2 library construction protocols:
- ABI's SOLiD Fragment Library Construction
- ABI's SOLiD Mate Pair Library Construction
- Illumina's Genome Analyzer Fragment Library Construction
- Illumina's Genome Analyzer Paired-end Library Construction
E-Gel precast agarose gels with SYBR safe gel stain
Experience safer analysis without gels to pour, buffers to make, or chemical staining. Invitrogen E-Gel Precast Agarose Gels with SYBR Safe Gel Stain are designed for safer, more convenient DNA electrophoresis.
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