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Gibco™
Trypsin (0.25%), phenol red
Gibco™ Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestiveRead more
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| Catalog Number | Quantity |
|---|---|
| 15050065 | 100 mL |
| 15050057 | 500 mL |
Catalog number 15050065
Price (USD)
19.65
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20.82Save 1.17 (6%)
Each
In stock
Quantity:
100 mL
Price (USD)
19.65
Online Exclusive
20.82Save 1.17 (6%)
Each
Gibco™ Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. We offer a variety of Gibco™ Trypsin and animal origin free TrypLE™ that feature:
• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing
The complete formulation is available.
Quality Testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.
Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco™ Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.
cGMP Manufacturing and Quality System
Gibco™ Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Trypsin solution made in our Scotland facility (25050-014). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing
This Trypsin is modified as follows:
| With | Without |
| • Phenol Red | • EDTA |
The complete formulation is available.
Quality Testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.
Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco™ Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.
cGMP Manufacturing and Quality System
Gibco™ Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco™ Trypsin solution made in our Scotland facility (25050-014). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsNo EDTA
Concentration1 X
Osmolality280 - 310 mOsm/kg
Product LineGibco™
Quantity100 mL
Shelf Life24 Months
Shipping ConditionWet Ice
SourceAnimal Origin
Tested ForIn vitro Bioassay
ClassificationAnimal Origin
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesPhenol Red
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Shelf life: 24 months from date of manufacture.
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3097463Certificate of AnalysisJun 06, 202515050065, 15050057
3203949Certificate of AnalysisJun 01, 202515050065, 15050057
3118226Certificate of AnalysisApr 26, 202515050065, 15050057
3011166Certificate of AnalysisFeb 14, 202515050065, 15050057
3011033Certificate of AnalysisJan 30, 202515050065, 15050057
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Frequently asked questions (FAQs)
Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Citations & References (2)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.
Journal:J Biol Chem
PubMed ID:11809764
Mammalian neural stem cells can develop into a variety of neuronal and glial cell types. This involves a highly coordinated process of differentiation in which the Notch signaling pathway and the system of helix-loop-helix (HLH) transcriptional regulators play a key role. By exercising control over proliferation, initiation of differentiation, neurite
Genetic Evidence That Protease-activated Receptors Mediate Factor Xa Signaling in Endothelial Cells.
Journal:J Biol Chem
PubMed ID:11850418
The coagulation protease Factor Xa (Xa)(1) triggers a variety of cellular responses that may be important for inflammatory reactions to tissue injury. Protease-activated receptors (PAR1, PAR2, and PAR4) can mediate Xa signaling in heterologous expression systems. However, other candidate Xa receptors have been described, and the extent to which one
2 total citations