Keratinocyte SFM (1X)
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Keratinocyte SFM (1X)
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Keratinocyte SFM (1X)

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cellsRead more
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Catalog NumberQuantity
17005042Promo Image500 mL
Catalog number 17005042
Price (USD)
152.65
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167.00
Save 14.35 (9%)
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Estimated availability date 17-Jul-2025
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Quantity:
500 mL
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Price (USD)
152.65
Online Exclusive
167.00
Save 14.35 (9%)
Each
Add to cart
Ask our AI about this Product
Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal substitutes, in vitro toxicology, and gene therapy.

Keratinocyte SFM is a complete serum-free medium when supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) at the time of use. Both supplements, rEGF and BPE, are supplied in separate packaging with the basal medium.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeKeratinocyte Cells, Epithelial Cells
Concentration1 X
Product TypeKit
Quantity500 mL
Shelf Life12 Months
ClassificationSerum-free
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelSerum-free
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red
Unit SizeEach
Contents & Storage
• 500 mL Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
• 25 mg Bovine Pituitary Extract (BPE); store in freezer (-5 to -20°C) and protect from light
• 2.5 μg EGF, Human Recombinant; store in freezer (-5 to -20°C) and protect from light

Complete Medium: store in refrigerator (2–8°C) and protect from light

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Frequently asked questions (FAQs)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the calcium concentration in Keratinocyte SFM (1X) (Cat. No. 17005042)?

Keratinocyte SFM (1X) (Cat. No. 17005042) includes Keratinocyte-SFM (Cat. No. 10724011) and Keratinocytes Supplements (Cat. No. 37000015), which contains Bovine Pituitary Extract (BPE) EGF, Human Recombinant. The complete media is prepared by adding the supplements to the base media. The Keratinocyte-SFM (Cat. No. 10724011) contains calcium chloride at 12.98 mg/L and D calcium pantothenate at 0.29 mg/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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2512119Certificate of AnalysisFeb 09, 202317005042, 170050025, 170051000, 170055000
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2487259Certificate of AnalysisFeb 09, 202317005042, 170050025, 170051000, 170055000
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