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Invitrogen™
Taq DNA Polymerase, native
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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| Catalog Number | Quantity |
|---|---|
| 18038042 | 500 Units |
| 18038018 | 100 Units |
| 18038067 | 3 x 500 Units |
| 18038240 | 5000 Units |
Catalog number 18038042
Price (USD)
252.85
Special offer
Online exclusive
Ends: 17-Jul-2025
389.00Save 136.15 (35%)
Each
-
Quantity:
500 Units
Price (USD)
252.85
Special offer
Online exclusive
Ends: 17-Jul-2025
389.00Save 136.15 (35%)
Each
Taq DNA Polymerase, native, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This native enzyme is purified from Thermus aquaticus YT1 and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.
Features
- A choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5 U/μL
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatStand-alone enzyme
Hot StartNo
No. of Reactions500 Reactions
Overhang3'-A
PolymeraseTaq DNA Polymerase
Product TypeDNA Polymerase
Quantity500 Units
Reaction FormatSeparate Components
Shipping ConditionWet or Dry Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
For Use With (Application)Standard PCR
GC-Rich PCR PerformanceLow
PCR MethodqPCR, Standard PCR
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C in a non-frost-free freezer.
Stable for 6 months when stored properly.
• 10X PCR buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C in a non-frost-free freezer.
Stable for 6 months when stored properly.
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Lot #Certificate TypeDateCatalog Number(s)
3210137Certificate of AnalysisMay 09, 202518038240
3150033Certificate of AnalysisApr 25, 202518038240
3107357Certificate of AnalysisFeb 07, 202518038018
3041360Certificate of AnalysisJan 27, 202518038042
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SDSCitations & References (25)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.
Journal:Methods Mol Biol
PubMed ID:19159105
'Over the past two decades, the field of biosensors has been developing fast, portable, and convenient detection tools for various molecules of interest, both biological and environmental. Although much attention is paid to the transduction portion of the sensor, the actual bioreceptor that binds the ligand is equally critical. Tight,
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Journal:J Biol Chem
PubMed ID:11773047
'We have determined previously that Sp3 encodes three distinct gene products as follows: a full-length protein (Sp3) that is an activator of transcription and two isoforms (M1 and M2) derived via internal translational initiation that function as transcriptional repressors. To identify amino acids and functions required for transcriptional repression, we
p38 alpha mitogen-activated protein kinase is activated by CD28- mediated signaling and is required for IL-4 production by human CD4+CD45RO+ T cells and Th2 effector cells.
Journal:J Immunol
PubMed ID:10358155
'T cell proliferation and cytokine production usually require stimulation via both the TCR/CD3 complex and the CD28 costimulatory receptor. Using purified human CD4+ peripheral blood T cells, we show that CD28 stimulation alone activates p38 alpha mitogen-activated protein kinase (p38 alpha). Cell proliferation induced by CD28 stimulation alone, a response
Role of de novo DNA methyltransferases and methyl CpG-binding proteins in gene silencing in a rat hepatoma.
Journal:J Biol Chem
PubMed ID:11844796
'The expression of metallothionein-I (MT-I), a known antioxidant, was suppressed in a transplanted rat hepatoma because of promoter methylation and was induced by heavy metals only after demethylation by 5-azacytidine (5-AzaC). Treatment of the tumor-bearing rats with 5-AzaC resulted in significant regression of the hepatoma. When the inhibitor-treated tumor was
Estrogen receptor alpha-mediated silencing of caveolin gene expression in neuronal cells.
Journal:J Biol Chem
PubMed ID:12138116
'Estrogen receptors (ER alpha/ER beta) are expressed in neuronal cells and exhibit a variety of activities in the central nervous system. ER activity is regulated in a ligand-dependent manner and by co-regulatory factors. Caveolin-1 is a recently identified co-activator of ER alpha mediating the ligand-independent activation of this steroid receptor.
25 total citations