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Gibco™
Trypsin-EDTA (0.25%), phenol red
This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixtureRead more
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| Catalog Number | Quantity |
|---|---|
| 25200072 | 500 mL |
| 25200056 | 100 mL |
| 25200114 | 20 x 100 mL |
Catalog number 25200072
Price (USD)
64.25
Each
In stock
Quantity:
500 mL
Price (USD)
64.25
Each
This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.
This trypsin solution is modified as follows:
With
• EDTA
• Phenol Red
The complete formulation is available.
We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.
Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.
Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.25%), phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
This trypsin solution is modified as follows:
With
• EDTA
• Phenol Red
The complete formulation is available.
We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.
Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.
Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Trypsin-EDTA (0.25%), phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Osmolality270 - 320 mOsm/kg
Product LineGibco™
Quantity500 mL
Shelf Life24 Months
Shipping ConditionWet Ice
SourceAnimal Origin
Tested ForIn vitro Bioassay
ClassificationAnimal Origin
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesPhenol Red, EDTA
pH7.1 to 8
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3224584Certificate of AnalysisJun 25, 202525200056, 25200072
3138532Certificate of AnalysisJun 22, 202525200056, 25200072
3209671Certificate of AnalysisJun 22, 202525200056, 25200072
3180927Certificate of AnalysisJun 22, 202525200056, 25200072
3126863Certificate of AnalysisJun 18, 202525200056, 25200072
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
We recommend storing the Trypsin-EDTA (0.25%), phenol red (Cat. No. 25200072) at -5°C to -20°C as specified in the Contents & Storage section of the Thermofisher Scientific product page for this product. Although it is possible to store the trypsin for up to one week at +4°C, we would recommend preparing aliquots and storing them at -5°C to -20°C. These aliquots should be used up after thawing and freeze/thaw cycles should be avoided.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Citations & References (4)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Electrophoretic profiling of both RNA and protein from a single 250-pL sample.
Journal:Anal Chem
PubMed ID:11985318
'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based
Generation of human induced pluripotent stem cells from urine samples.
Journal:Nat Protoc
PubMed ID:23138349
'Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method
Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
Journal:Nature
PubMed ID:23041930
One goal of regenerative medicine, to use stem cells to replace cells lost by injury or disease, depends on producing an excess of the relevant cell for study or transplantation. To this end, the stepwise differentiation of stem cells into specialized derivatives has been successful for some cell types, but
Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.
Journal:J Biol Chem
PubMed ID:8631880
The precursor of matrix metalloproteinase 3 (MMP-3/ stromelysin 1) is activated in vitro by proteinases or mercurial compounds by stepwise processes which include the initial formation of short-lived intermediates and the subsequent intermolecular cleavage of the His82-Phe83 bond to generate the fully activated mature MMP-3 (Nagase, H., Enghild, J. J.,
4 total citations