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Citations & References (23)
Gibco™
Penicillin-Streptomycin (5,000 U/mL)
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined actionRead more
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| Catalog Number | Quantity |
|---|---|
| 15070063 | 100 mL |
Catalog number 15070063
Price (CNY)
430.00
Each
In stock
Quantity:
100 mL
Price (CNY)
430.00
Each
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria. This solution contains 5000 units/mL of penicillin and 5000 μg/mL of streptomycin.
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection
See recommended working concentrations for selection antibiotics.
Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. See the complete list, or find products for:
• Contamination control
• Eukaryotic and bacterial selection
See recommended working concentrations for selection antibiotics.
Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 X
Culture TypeMammalian Cell Culture, Insect Cell Culture
For Use With (Application)Prevention of Cell Culture Contamination
Quantity100 mL
Shelf Life12 Months
Shipping ConditionDry Ice
FormLiquid
Product TypeAntibiotic
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
247320Certificate of AnalysisMar 23, 202515070063
2588018Certificate of AnalysisJan 29, 202515070063
2779735Certificate of AnalysisJan 29, 202515070063
2779736Certificate of AnalysisJan 29, 202515070063
2588019Certificate of AnalysisJan 29, 202515070063
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SDSScientific Resources
Frequently asked questions (FAQs)
Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (23)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion
A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.
Journal:J Biol Chem
PubMed ID:11786532
'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in
Electrophoretic profiling of both RNA and protein from a single 250-pL sample.
Journal:Anal Chem
PubMed ID:11985318
'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based
23 total citations