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Applied Biosystems™
AmpliTaq Gold™ 360 Master Mix
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
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| Catalog Number | Quantity |
|---|---|
| 4398901 | 10 x 5 mL |
| 4398876 | 1 mL |
| 4398881 | 5 mL |
| 4398886 | 50 mL |
Catalog number 4398901
Price (HKD)
15,753.00
Each
Quantity:
10 x 5 mL
Price (HKD)
15,753.00
Each
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package with all components premixed and premeasured. Designed for convenience, this master mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase, but it also contains the world-class 360 GC Enhancer, which can be added for high GC-content templates. With the wide template coverage, optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix.
Features
- Optimized for a broad range of targets
- High sensitivity, specificity, and yield
- Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
- Helps achieves high-quality sequencing data
- Easy-to-use, premixed master mix
Optimized for easy and challenging targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions. Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as a high-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets.
Optimized for automated, hot-start PCR
AmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. The extreme specificity allows easier multiplexing and allelic discrimination.
Amplify low-copy amplicons and long targets
AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences.
Notes
- AmpliTaq Gold 360 PCR Master Mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
Exonuclease Activity5' - 3'
Fidelity (vs. Taq)1X
FormatTube
Green FeaturesSustainable packaging
Hot StartBuilt-In Hot Start
No. of Reactions2000 Reactions
Overhang3'-A
PolymeraseAmpliTaq Gold 360 DNA Polymerase
Product TypePCR Master Mix
Quantity10 x 5 mL
Reaction FormatSuperMix or Master Mix
Shipping ConditionRoom Temp or Wet Ice
Size (Final Product)5 kb or less
Starting MaterialDNA
Volume50 mL
For Use With (Application)Cloning, Hot-start PCR
GC-Rich PCR PerformanceHigh
Reaction SpeedStandard
Unit SizeEach
Contents & Storage
• 2X AmpliTaq Gold 360 Master Mix, 10 x 5 mL
• 360 GC Enhancer, 10 x 1.5 mL
Store at -20°C until first use, then store at 4°C.
• 360 GC Enhancer, 10 x 1.5 mL
Store at -20°C until first use, then store at 4°C.
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Figures
Average specific yield and specificity for AmpliTaq Gold® 360 Taq® DNA Polymerase compared to other enzymes.
High-quality sequencing data obtained from AmpliTaq Gold® 360 DNA Polymerase-generated PCR products.
Amplification of long targets with AmpliTaq Gold® 360 DNA Polymerase.
Sensitivity of AmpliTaq Gold® 360 DNA Polymerase for detection of low copy targets.
AmpliTaq Gold® 360 DNA Polymerase demonstrates superior sensitivity and specific yield.
AmpliTaq Gold® 360 Master Mix Amplifies a broad range of targets.
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Lot #Certificate TypeDateCatalog Number(s)
3262506Certificate of AnalysisJun 17, 20254398881
3257846Certificate of AnalysisJun 10, 20254398876
3254816Certificate of AnalysisJun 05, 20254398881
3254869Certificate of AnalysisJun 05, 20254398881
3255124Certificate of AnalysisJun 05, 20254398876
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Product Information
Frequently asked questions (FAQs)
Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.
Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.
The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.
If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.
If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.
Citations & References (1)
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Citations & References
Abstract
Identification of rodent homologs of hepatitis C virus and pegiviruses.
Journal:MBio
PubMed ID:23572554
Hepatitis C virus (HCV) and human pegivirus (HPgV or GB virus C) are globally distributed and infect 2 to 5% of the human population. The lack of tractable-animal models for these viruses, in particular for HCV, has hampered the study of infection, transmission, virulence, immunity, and pathogenesis. To address this
1 total citations