Trypsin/EDTA Solution (TE)
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Gibco™

Trypsin/EDTA Solution (TE)

Gibco™ Trypsin solutions are made from trypsin powder - a mixture of proteases derived from bovine pancreas. Due to itsRead more
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Catalog NumberQuantity
R001100Promo Image100 mL
Catalog number R001100
Price (HKD)
376.00
Each
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Quantity:
100 mL
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Price (HKD)
376.00
Each
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Gibco™ Trypsin solutions are made from trypsin powder - a mixture of proteases derived from bovine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. This Gibco™ Trypsin/EDTA solution is formulation as 0.025% trypsin and 0.01% EDTA in Phosphate Buffered Saline (PBS). We offer a variety of Gibco™ Trypsin and animal origin free TrypLE™ cell dissociation products to meet your needs.

This trypsin is manufactured as follows:

With
EDTA
Phenol Red
The complete formulation is available.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco™ Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
ChelatorsEDTA
Concentration1 X
Product LineCascade Biologics™
Quantity100 mL
Shelf Life18 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
With AdditivesPhenol Red, EDTA
pH7.4
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -20°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3011100Certificate of AnalysisMar 06, 2025R001100
2425734Certificate of AnalysisJan 29, 2025R001100
2565092Certificate of AnalysisJan 29, 2025R001100
2565098Certificate of AnalysisJan 29, 2025R001100
2565094Certificate of AnalysisJan 29, 2025R001100
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Safety Data Sheets

Frequently asked questions (FAQs)

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

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