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SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)
SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)
Invitrogen™

SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

SYBR™ Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for the detection of nucleic acids.자세히 알아보기
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카탈로그 번호수량
S11494Promo Image500 μL
카탈로그 번호 S11494
제품 가격(KRW)
273,000
Online offer
Ends: 30-Sep-2025
287,000
할인액 14,000 (5%)
Each
재고 있음
카트에 추가하기
수량:
500 μL
Recurring order eligible. Learn more »
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
273,000
Online offer
Ends: 30-Sep-2025
287,000
할인액 14,000 (5%)
Each
카트에 추가하기
Ask our AI about this Product
SYBR™ Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for the detection of nucleic acids. Use with UV transilluminators, the Dark Reader (see stained nucleic acid gel below), laser scanners, or blue light transilluminators such as the Safe Imager™ 2.0 or the E-Gel™ Imager.

Features of SYBR™ Gold Nucleic Acid Gel Stain:

• Ultra Sensitive: 25–100 times more sensitive than ethidium bromide. Detect as little as 25 pg of DNA
• Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage
• Increased Signal to Background Ratio: More than 1000-fold signal enhancement when bound to nucleic acids
• Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels

Stain Properties
SYBR™ Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (∼0.6) upon binding to double- or single-stranded DNA or to RNA1. Excitation maxima for dye-nucleic acid complexes are at ∼495 nm and ∼300 nm and the emission maximum is ∼537 nm (see spectra).

Stain Sensitivity
SYBR™ Gold stain is >10-fold more sensitive than ethidium bromide for detecting DNA and RNA in denaturing urea, glyoxal, and formaldehyde gels, even with 300 nm transillumination (see visualization of glyoxalatd RNA below). SYBR™ Gold stain has also been shown to be much more sensitive than SYBR™ Green II stain for detecting single strand conformation polymorphism (SSCP) products (see figure below). Furthermore, it is much more sensitive than silver-staining for the detection of double-stranded DNA in native polyacrylamide gels (see sensitivity comparison below).

1Tuma RS, Beaudet MP, Jin X, Jones LJ, Cheung CY, Yue S, Singer VL. Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem (1999) 268:278-288. (PMID: 10075818)
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 위치In-Gel Detection
검출 방법Fluorescence
제품 유형Nucleic Acid Gel Stain
수량500 μL
배송 조건Room Temperature
타겟 분자RNA, DNA
라벨 또는 염료SYBR Gold
Unit SizeEach
구성 및 보관
• Supplied as a 10,000X concentrate in DMSO

Store at -20°C, protected from light in a dessicator.
Have questions about this product? Ask our AI assisted search.
How should I dispose of SYBR DNA Gel Stain?
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Can I use the ethidium bromide filters on my camera to image SYBR dyes?
Can I detect a 5-nucleotide long ssDNA with SYBR Gold Nucleic Acid Gel Stain (Cat. No. S11494)?
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그림

형광 스펙트럼

Fluorescence spectra

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문서 및 다운로드

Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
2983236Certificate of Analysis2024년 9월 24일S11494
2901569Certificate of Analysis2024년 5월 03일S11494
2700997Certificate of Analysis2023년 8월 25일S11494
2549279Certificate of Analysis2023년 1월 10일S11494
2480131Certificate of Analysis2022년 5월 24일S11494
시험 성적서 관련 5 개의 결과가 검색되었습니다.

Safety Data Sheets | 물질 안전 보건 자료

자주 묻는 질문(FAQ)

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

인용 및 참조 문헌 (60)

인용 및 참조 문헌
Abstract
Authors:
Journal:
PubMed ID:16517648
The transcriptional coactivator CREB-binding protein cooperates with STAT1 and NF-kappa B for synergistic transcriptional activation of the CXC ligand 9/monokine induced by interferon-gamma gene.
Authors:Hiroi M, Ohmori Y
Journal:J Biol Chem
PubMed ID:12403783
'Signal transducers and activators of transcription 1 (STAT1) and NF-kappaB cooperatively regulate the expression of many inflammatory genes. In the present study, we demonstrate that the transcriptional coactivator CREB-binding protein (CBP) mediated the STAT1/NF-kappaB synergy for transcription of the gene for CXC ligand 9 (CXCL9), an interferon-gamma (IFN-gamma)-inducible chemokine. Reporter ... More
Gel staining methods for detection of telomerase activity with the telomeric repeat amplification protocol (TRAP) assay.
Authors:Fujita M, Tomita S, Ueda Y, Fujimori T
Journal:Mol Pathol
PubMed ID:10193516
DOTAP cationic liposomes prefer relaxed over supercoiled plasmids.
Authors:Even-Chen S, Barenholz Y
Journal:Biochim Biophys Acta
PubMed ID:11118529
'Cationic liposomes and DNA interact electrostatically to form complexes called lipoplexes. The amounts of unbound (free) DNA in a mixture of cationic liposomes and DNA at different cationic lipid:DNA molar ratios can be used to describe DNA binding isotherms; these provide a measure of the binding efficiency of DNA to ... More
Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold.
Authors:Chen F, Lu JR, Binder BJ, Liu YC, Hodson RE
Journal:Appl Environ Microbiol
PubMed ID:11157214
'A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples. Viral particles stained with SYBR Gold yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry. The fluorescent signal strength of ... More
60 total citations

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