The purpose of a buffer in a biological system is to maintain intracellular and extracellular pH within the physiological range, as well as to resist changes in pH due to the presence of internal and external influences. Thermo Scientific offers a selection of ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Good and co-workers in 1966, these organic buffers have pKa values at or near physiological pH and minimally interfere with biological processes. They are suitable for biochemical research applications such as cell cultivation, isolation of cells, enzyme assays, and more.

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Obtaining optimal results in your research requires careful selection of reagents. Our life science buffers are manufactured from high-quality raw materials, sterilized by submicron filtration and/or autoclaving, and tested under ISO 9001 2000-certified manufacturing standards to provide the lot-to-lot consistency and reliability you’ve come to expect with Thermo Scientific products. 

Advantages

  • Cell culture tested
  • Analyzed for the absence of nucleases and proteases
  • Tested for endotoxin and bioburden levels
  • pKa values mostly independent of temperature and concentration
  • High water solubility
  • Minimal permeability to biological membranes

Laboratory applications

  • Tissue culture media and maintenance of cell lines
  • Enzyme assays
  • Electrophoresis of RNA
  • Transfection of mammalian cells


Ordering information

Catalog # Name Size Price (USD) Qty
043496.14 1,3-Bis[tris(hydroxymethyl)methylamino]propane, 98+% Each
71.65

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79.30
Save 7.65 (10%)
043496.22 1,3-Bis[tris(hydroxymethyl)methylamino]propane, 98+% Each
234.65

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260.00
Save 25.35 (10%)
043496.36 1,3-Bis[tris(hydroxymethyl)methylamino]propane, 98+% Each
946.65

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Ends: 26-Sep-2025

1,114.00
Save 167.35 (15%)
B22515.14 2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 98% Each
36.65

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Ends: 30-Jun-2025

43.50
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B22515.22 2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 98% Each
90.65

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Ends: 30-Jun-2025

107.00
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B22515.36 2,2-Bis(hydroxymethyl)-2,2',2″-nitrilotriethanol, 98% Each
346.65

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Ends: 26-Sep-2025

408.00
Save 61.35 (15%)
J61821.AK MOPS, 1.0M buffer soln., pH 7.0 Each
141.65

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157.00
Save 15.35 (10%)
J61821.AP MOPS, 1.0M buffer soln., pH 7.0 Each
225.65

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Ends: 26-Sep-2025

265.00
Save 39.35 (15%)
J61196.AP Phosphate-buffered saline (PBS, 1X), sterile-filtered Each
42.50
J60893.K2 Phosphate-buffered saline (PBS, 5X), with EDTA Each
69.65

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Online exclusive

Ends: 30-Jun-2025

81.20
Save 11.55 (14%)
J60893.K3 Phosphate-buffered saline (PBS, 5X), with EDTA Each
99.65

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Ends: 30-Jun-2025

117.00
Save 17.35 (15%)
J62885.AD RIPA buffer with Triton∣r X-100 (5X) Each
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J62885.AE RIPA buffer with Triton∣r X-100 (5X) Each
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J61367.AE SM buffer, pH 7.5 Each
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J61367.AK SM buffer, pH 7.5 Each
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J63677.K2 TAE (10X), TRIS + acetate + EDTA Each
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J63677.K3 TAE (10X), TRIS + acetate + EDTA Each
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J63677.K7 TAE (10X), TRIS + acetate + EDTA Each
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J63931.K2 TAE (50X), TRIS + acetate + EDTA Each
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J63931.K3 TAE (50X), TRIS + acetate + EDTA Each
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J63931.K7 TAE (50X), TRIS + acetate + EDTA Each
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J62552.AP TRIS, 0.5M buffer soln., pH 8.0 Each
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J62552.K2 TRIS, 0.5M buffer soln., pH 8.0 Each
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J16924.AE HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure Each
71.20
J16924.AP HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure Each
147.65

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Ends: 30-Jun-2025

173.00
Save 25.35 (15%)
J16924.K2 HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure Each
249.65

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Ends: 26-Sep-2025

294.00
Save 44.35 (15%)
J17525.22 Imidazole, >98.5% (Dry Basis), Ultrapure Each
68.65

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Ends: 30-Jun-2025

80.50
Save 11.85 (15%)
J17525.36 Imidazole, >98.5% (Dry Basis), Ultrapure Each
119.65

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Ends: 26-Sep-2025

141.00
Save 21.35 (15%)
J17525.A1 Imidazole, >98.5% (Dry Basis), Ultrapure Each
200.65

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Ends: 30-Jun-2025

236.00
Save 35.35 (15%)
J17525.A7 Imidazole, >98.5% (Dry Basis), Ultrapure Each
824.65

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916.00
Save 91.35 (10%)
J18886.22 MES Monohydrate, ≥98.5% (Dry Basis), Ultrapure Each
108.65

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Ends: 26-Sep-2025

128.00
Save 19.35 (15%)
J18886.A1 MES Monohydrate, ≥98.5% (Dry Basis), Ultrapure Each
452.65

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Ends: 26-Sep-2025

532.00
Save 79.35 (15%)
J19256.22 MOPS, ≥98.5% (Dry Basis), Ultrapure Each
79.65

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88.50
Save 8.85 (10%)
J19256.A1 MOPS, ≥98.5% (Dry Basis), Ultrapure Each
294.65

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Ends: 30-Jun-2025

346.00
Save 51.35 (15%)
J75889.AE PBS, 10X Solution, Molecular Biology Grade Each
47.40
J75889.K2 PBS, 10X Solution, Molecular Biology Grade Each
87.65

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Online exclusive

Ends: 26-Sep-2025

103.00
Save 15.35 (15%)
J75889.K8 PBS, 10X Solution, Molecular Biology Grade Each
157.65

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Ends: 30-Jun-2025

185.00
Save 27.35 (15%)
J75892.AE TBS, 20X Solution, pH 7.4, Molecular Biology Grade, Ultrapure Each
62.65

Online Exclusive

69.20
Save 6.55 (9%)
J75892.K2 TBS, 20X Solution, pH 7.4, Molecular Biology Grade, Ultrapure Each
112.65

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Ends: 30-Jun-2025

132.00
Save 19.35 (15%)
J75892.K8 TBS, 20X Solution, pH 7.4, Molecular Biology Grade, Ultrapure Each
199.65

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Ends: 26-Sep-2025

235.00
Save 35.35 (15%)
J77500.K2 TBS with Tween™ (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Each
187.65

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Ends: 30-Jun-2025

220.00
Save 32.35 (15%)
J77500.K8 TBS with Tween™ (TBST), 20X Solution, Molecular Biology Grade, Ultrapure Each
376.65

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418.00
Save 41.35 (10%)
J75893.AE TE Buffer, 1X Solution, Molecular Biology Grade, Ultrapure Each
69.65

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Ends: 26-Sep-2025

81.80
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J75893.AP TE Buffer, 1X Solution, Molecular Biology Grade, Ultrapure Each
90.65

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101.00
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J75793.AE TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade Each
60.65

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Ends: 26-Sep-2025

71.00
Save 10.35 (15%)
J75793.AP TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade Each
101.65

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113.00
Save 11.35 (10%)
J22561.22 Tricine, ≥98.5% (Dry Basis), Ultrapure Each
64.80
J22561.36 Tricine, ≥98.5% (Dry Basis), Ultrapure Each
251.00
J22561.A1 Tricine, ≥98.5% (Dry Basis), Ultrapure Each
367.00
J22675.36 Tris, ≥98% Each
91.00
J22675.A1 Tris, ≥98% Each
116.00
J22674.36 Tris, 99.0 to 101.0% (Dry Basis) Each
85.60
J22674.A1 Tris, 99.0 to 101.0% (Dry Basis) Each
119.00
J22674.A7 Tris, 99.0 to 101.0% (Dry Basis) Each
418.00
J22638.AE Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure Each
43.70
J22638.AP Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure Each
68.00
J22638.K2 Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure Each
84.70

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