Search Thermo Fisher Scientific
In Vitro Transcription—Thermo Scientific
Accelerating in vitro RNA synthesis
In vitro transcription is a straightforward procedure to synthesize RNA from DNA by using components of bacteriophage systems; T7, T3 or SP6 systems. Thermo Scientific offers high quality and efficient tools for RNA synthesis, available in complete kits or stand-alone products (e.g., RNA polymerases, dNTPs, etc.). In vitro transcripts are suitable for many applications including RNA functional (e.g., aptamers and ribozymes) and structural studies, probes in analytical techniques (e.g., hybridization analysis), biochemical and genetic studies (e.g., antisense reagents).
Broad portfolio of products covering the complete RNA synthesis workflow
| In vitro transcription | Post-transcriptional modification | Template degradation | |
|---|---|---|---|
| Stand-along products | |||
| Transcription kits |
RNA synthesis kits
TranscriptAid T7 High Yield Transcription Kit is designed for high yield in vitro transcription (short and long fragments) using T7 RNA Polymerase. All necessary reagents for transcription are included.
Highlights
- Exceptionally high yields – up to 200 ug RNA transcripts in 2 hours and mg level of RNA in a single scaled-up reaction
- Versatile – suitable for short and long RNA transcripts
- Flexible – generates unlabeled, labeled or capped RNA
- Sizing and quantification – RiboRuler RNA ladder supplied with kit for verification
RNA synthesis enzymes
RNA polymerases and post-transcriptional modification enzymes for in-vitro transcription.
Buffers & reagents
High quality dilution buffers and concentration reagents for RNA synthesis and electrophoresis.
Molecular biology grade dNTPs can be used in all demanding molecular biology applications and are highly stable with purity greater than 99% (confirmed by HPLC).
Resources
Molecular Biology Resource Library
Webinars, videos, articles, and selection guides
Molecular Biology Web Tools
Tools for primer analysis, reaction setup, biochemical conversions, and calculators