MagMAX™-96 Total RNA Isolation Kit
MagMAX™-96 Total RNA Isolation Kit
Invitrogen™

MagMAX™-96 Total RNA Isolation Kit

The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cellsRead more
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Catalog NumberQuantity
AM183096 Preps
Catalog number AM1830
Price (USD)
548.00
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Quantity:
96 Preps
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Price (USD)
548.00
Each
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The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions. Features of the MagMAX™-96 Total RNA Isolation Kit include:

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput users

Benefits of MagMAX™ magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.

Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10 mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit, and downloadable protocols for the use of this kit with specific liquid handling systems are available at the Automation Resource.

Accessory products
Lysis/Binding Solution Concentrate (AM8500), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid (AM9690) can be used in conjunction with the MagMAX™-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume50 μL
Final Product TypeTotal RNA
For Use With (Application)RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
For Use With (Equipment)KingFisher™ Duo Prime, KingFisher™ Flex, Automated Liquid Handling Systems
High-throughput CompatibilityHigh-throughput Compatible
Quantity96 Preps
Shipping ConditionBox 1: Dry Ice
Box 2: Room Temperature
Starting Material AmountCells: ≤106
Plant: ≤10 mg
Tissue: ≤5 mg
Yield≤20 pg per cell
Isolation TechnologyMagnetic Bead
Sample TypeCells, Plants, Tissue
Unit SizeEach
Contents & Storage
• 1 Processing Plate; room temperature
• 11 mL Lysis/Binding Solution (concentrated); room temperature
• 18 mL Wash Solution 1 (concentrated); room temperature
• 55 mL Wash Solution 2 (concentrated); room temperature
• 12 mL RNA Rebinding (concentrated); room temperature
• 10 mL Elution Buffer; room temperature
• 6 mL MagMAX TURBO DNase Buffer; room temperature
• 1.1 mL RNA Binding Beads; 4°C
• 1.1 mL Lysis/Binding Enhancer; -20°C
• 110 μL TURBO DNase; -20°C

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
2710108Certificate of AnalysisApr 26, 2023AM1830
2708901Certificate of AnalysisApr 24, 2023AM1830
2700125Certificate of AnalysisApr 12, 2023AM1830
2693622Certificate of AnalysisMar 31, 2023AM1830
2692528Certificate of AnalysisMar 31, 2023AM1830
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Safety Data Sheets

Frequently asked questions (FAQs)

Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed. High salt carry over is not optimal for MagMax RNA isolation steps.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

For the MagMAX-96 AI/ND Viral RNA Isolation Kit and MagMAX-96 Total RNA Isolation Kit, we recommend using an orbital shaker for bead mixing and washing. We recommend the following orbital shaker:
Digital Microplate Shakers

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Citations & References (6)

Citations & References
Abstract
Parkinson's disease-linked leucine-rich repeat kinase 2(R1441G) mutation increases proinflammatory cytokine release from activated primary microglial cells and resultant neurotoxicity.
Authors:Gillardon F, Schmid R, Draheim H
Journal:Neuroscience
PubMed ID:22342962
'Mutations in leucine-rich repeat kinase 2 (LRRK2) have been causally linked to neuronal cell death in Parkinson''s disease. LRRK2 expression has also been detected in B lymphocytes and macrophages, suggesting a role in immune responses. In the present study, we demonstrate that LRRK2 is expressed in primary microglial cells isolated ... More
Effects of interleukin-6 (IL-6) and an anti-IL-6 monoclonal antibody on drug-metabolizing enzymes in human hepatocyte culture.
Authors:Dickmann LJ, Patel SK, Rock DA, Wienkers LC, Slatter JG
Journal:Drug Metab Dispos
PubMed ID:21555507
'The cytokine-mediated suppression of hepatic drug-metabolizing enzymes by inflammatory disease and the relief of this suppression by successful disease treatment have recently become an issue in the development of drug interaction labels for new biological products. This study examined the effects of the inflammatory cytokine interleukin-6 (IL-6) on drug-metabolizing enzymes ... More
Newly recognized bocaviruses (HBoV, HBoV2) in children and adults with gastrointestinal illness in the United States.
Authors:Chow BD, Ou Z, Esper FP
Journal:J Clin Virol
PubMed ID:20036617
The human bocavirus (HBoV) is a newly recognized parvovirus associated with respiratory and gastrointestinal disease. Recently, two new members of the parvovirus family have been recognized, HBoV2 and HBoV3. ... More
Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy.
Authors:Archin NM, Liberty AL, Kashuba AD, Choudhary SK, Kuruc JD, Crooks AM, Parker DC, Anderson EM, Kearney MF, Strain MC, Richman DD, Hudgens MG, Bosch RJ, Coffin JM, Eron JJ, Hazuda DJ, Margolis DM
Journal:Nature
PubMed ID:22837004
Despite antiretroviral therapy, proviral latency of human immunodeficiency virus type 1 (HIV-1) remains a principal obstacle to curing the infection. Inducing the expression of latent genomes within resting CD4(+) T cells is the primary strategy to clear this reservoir. Although histone deacetylase inhibitors such as suberoylanilide hydroxamic acid (also known ... More
Immiscible phase nucleic acid purification eliminates PCR inhibitors with a single pass of paramagnetic particles through a hydrophobic liquid.
Authors:Sur K, McFall SM, Yeh ET, Jangam SR, Hayden MA, Stroupe SD, Kelso DM
Journal:J Mol Diagn
PubMed ID:20581047
Extraction and purification of nucleic acids from complex biological samples for PCR are critical steps because inhibitors must be removed that can affect reaction efficiency and the accuracy of results. This preanalytical processing generally involves capturing nucleic acids on microparticles that are then washed with a series of buffers to ... More
6 total citations

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