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Lyo-Ready Enzymes for Molecular Diagnostics
Thermo Fisher Scientific offers new-generation enzymes in lyo-ready or lyophilization-compatible format. Lyophilization also known as freeze-drying is a process that involves removing the water content from a substance by freezing it and then rapidly evaporating the frozen water under reduced pressure. This technique helps to preserve perishable products while maintaining their structure and extending their shelf life. Enzymes in lyo-ready format are portable, room-temperature stable, and suitable for molecular assay development. Lyo-ready enzymes retain characteristics of conventional enzyme including reproducibility, sensitivity, and specificity required for diagnostic assays.
Benefits of lyo-ready enzymes
- Flexible assay designs maximize the same functional enzyme performance with glycerol content below 0.1–0.5%
- Compatibility with microfluidics solutions, such as point-of-care (POC) testing, single-cell analysis, and droplet-based techniques
- High confidence in results with minimal risk of bacterial, human, or plasmid DNA contamination
- Assurance of ISO 13485 quality
- Lot-to-lot consistency
Lyo-ready enzymes: The solution for POC molecular diagnostics
Lyo-ready enzymes hold promise and potential for faster and accurate point of care diagnostics. According to an article published in Nature , these 'lyo-ready' enzymes can be used to build dried mixtures of PCR reagents that enable sample-to-answer assays. Thermo Fisher Scientific offers customized solutions for specific applications, including modified concentrations, volume, and packaging.
Video: The evolution of lyophilization
Portfolio of lyo-ready enzymes
Explore lyo-ready enzymes for reverse transcription, isothermal amplification, PCR amplification, and other related technologies.
| Product | Applicable technology |
|---|---|
| Lyo-ready SuperScript III Flash | 1-step RT-qPCR |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR | 1-step RT-qPCR |
| Invitrogen SuperScript III RT | 1-step RT-qPCR |
| Invitrogen SuperScript IV RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific M-MLV RT | 1-step RT-qPCR |
| Thermo Scientific RevertAid H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, RT-LAMP, cDNA synthesis |
| Thermo Scientific RevertAid M-MLV RT | 1-step RT-qPCR |
View the enzyme selection guide to select products suitable for your application
| Product | Applicable technology |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Bst DNA Polymerase | LAMP, HAD, EXPAR, RCA, RPA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Product | Applicable technology |
|---|---|
| Invitrogen Platinum II Taq DNA polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Invitrogen Platinum Taq DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Applied Biosystems AmpliTaq Gold DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Invitrogen LibertyTaq DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Thermo Scientific Phire Hot Start II DNA Polymerase | PCR |
View the enzyme selection guide to select products suitable for your application
| Product | Applicable technology |
|---|---|
| Invitrogen RNaseOUT RNase inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Thermo Scientific RiboLock RNase Inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Product | Applicable technology |
|---|---|
| Lyo-ready SuperScript III Flash | 1-step RT-qPCR |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR | 1-step RT-qPCR |
| Invitrogen SuperScript III RT | 1-step RT-qPCR |
| Invitrogen SuperScript IV RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific Maxima RT | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis |
| Thermo Scientific M-MLV RT | 1-step RT-qPCR |
| Thermo Scientific RevertAid H Minus RT | 2-step RT-qPCR, 1-step RT-PCR, RT-LAMP, cDNA synthesis |
| Thermo Scientific RevertAid M-MLV RT | 1-step RT-qPCR |
View the enzyme selection guide to select products suitable for your application
| Product | Applicable technology |
|---|---|
| RPA proteins and reagents | RPA |
| Invitrogen Bst DNA Polymerase | LAMP, HAD, EXPAR, RCA, RPA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Product | Applicable technology |
|---|---|
| Invitrogen Platinum II Taq DNA polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Invitrogen Platinum Taq DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Applied Biosystems AmpliTaq Gold DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Invitrogen LibertyTaq DNA Polymerase | 2-step RT-qPCR, 1-step RT-qPCR |
| Thermo Scientific Phire Hot Start II DNA Polymerase | PCR |
View the enzyme selection guide to select products suitable for your application
| Product | Applicable technology |
|---|---|
| Invitrogen RNaseOUT RNase inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
| Thermo Scientific RiboLock RNase Inhibitor | 2-step RT-qPCR, 1-step RT-PCR, LAMP, cDNA synthesis, NASBA, TMA |
Lyo-ready enzymes commonly used in assay development
The commonly used lyo-ready enzymes in molecular diagnostic assay development are reverse transcriptases, DNA polymerases, and isothermal enzymes.
Lyo-ready enzymes: Success stories
Our customers have successfully used lyo-ready Maxima reverse transcriptase, lyo-ready Bsm polymerase, and lyo-ready Platinum Taq DNA polymerase in assay development. Hear from them.
“It has been very useful for stabilizing the assay without a need to concentrate the enzyme in an effort to minimize glycerol level."
—R&D Manager, Small Business Health Care Company
Customer used lyo-ready Maxima reverse transcriptase in assay development for robust amplification of low-purity and degraded input RNA.
“Providing robust glycerol-free enzymes that performed in our assays as required without the need for big changes in formulations/processes. Providing reliable technical support and flexible supply service.”
—R&D Manager, Medium Enterprise Health Care Company
Customer used lyo-ready Bsm polymerase in assay development which improved the performance of the assay.
“They have performed better than other enzymes, this has allowed us more robust amplification.”
—R&D Director, Small Business Consumer Products Company
Customer integrated lyo-ready Platinum Taq DNA polymerase into existing assay development protocol.
Read the full case study on Lyo-ready Platinum Taq DNA polymerase
Resources for lyo-ready enzymes
Browse our collection of selection guides, technical articles, manuals, and protocols on lyo-ready enzymes.
Webinar
Manuals and protocols
Selection guides
Technical articles
FAQs
Enzymes in lyo-ready format are beneficial when portable, room-temperature stable assays are being developed. Lyo-ready enzymes retain all conventional enzyme characteristics like reproducibility, sensitivity, and specificity required for diagnostic assays.
We focus on supplying the raw materials for your assay development. All our enzymes come in liquid form compatible for lyophilization. We do not offer dried down enzymes nor lyophilization sevices.
The SuperScript IV RT-LAMP Master Mix is available for commercial use. It is optimized for best results in LAMP and RT-LAMP in glycerol format, lyo-ready mastermix is not available.
Nevertheless, we offer lyo-ready components of the master mix—the SSIV reverse transcriptase, Bst DNA polymerase, and RNaseOUT RNase inhibitor—as well as user manual for LAMP reaction setup. These components are available in a glycerol-free format and can be customized further upon request.
Resources
Check out our resources on molecular assay development.
Technical literature
Access technical and application notes on molecular cloning.
Webinars
Stay up-to-date on industry trends in molecular diagnostics with on-demand webinars from industry experts.
Digital handbooks
Review content on workflow, troubleshooting, strategies, and selection guides.
Support and services
Get support for your molecular assay development. For information on a personalized IVD transition plan contact us at ivdrtransition@thermofisher.com
For Research Use Only. Not for use in diagnostic procedures.
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