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There are various sizes of dishes and flasks used for cell culture. Some useful cell culture numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.
Cat. No. | Surface area (cm2) | Seeding density* | Cells at confluency† | Versene (mL of 0.05% EDTA), approx. volume | Trypsin (mL of 0.05% trypsin, 0.53 mM EDTA), approx. volume | Growth medium (mL), approx. volume | |
---|---|---|---|---|---|---|---|
Dishes | |||||||
35 mm | 150460 150318 | 8.8 | 0.3 x 106 | 1.2 x 106 | 1 | 1 | 2 |
60 mm | 150462 150288 | 21.5 | 0.8 x 106 | 3.2 x 106 | 3 | 3 | 5 |
100 mm | 150464 150350 | 56.7 | 2.2 x 106 | 8.8 x 106 | 5 | 5 | 12 |
150 mm | 150468 168381 | 145 | 5.0 x 106 | 20.0 x 106 | 10 | 10 | 30 |
Culture plates | |||||||
6-well | 140675 | 9.6 | 0.3 x 106 | 1.2 x 106 | 1 | 1 | 1 to 3 |
12-well | 150628 | 3.5 | 0.1 x 106 | 0.5 x 106 | 0.4 to 1 | 0.4 to 1 | 1 to 2 |
24-well | 142475 | 1.9 | 0.05 x 106 | 0.24 x 106 | 0.2 to 0.3 | 0.2 to 0.3 | 0.5 to 1.0 |
48-well | 150687 | 1.1 | 0.03 x 106 | 0.12 x 106 | 0.1 to 0.2 | 0.1 to 0.2 | 0.2 to 0.4 |
96-well | 167008 | 0.32 | 0.01 x 106 | 0.04 x 106 | 0.05 to 0.1 | 0.05 to 0.1 | 0.1 to 0.2 |
384-well | 164688 | 0.084 | 0.0018 x 106 | 0.0072 x 106 | 0.015 | 0.015 | 0.08 |
1536-well | 253614 | 0.03 | 0.0009 x 106 | 0.0036 x 106 | 0.003 | 0.003 | 0.01 |
Flasks | |||||||
T-25 | 156367 156340 | 25 | 0.7 x 106 | 2.8 x 106 | 3 | 3 | 3 to 5 |
T-75 | 156499 156472 | 75 | 2.1 x 106 | 8.4 x 106 | 5 | 5 | 8 to 15 |
T-175 | 159910 159920 | 175 | 4.9 x 106 | 23.3 x 106 | 17 | 17 | 35 to 53 |
T-225 | 159934 159933 | 225 | 6.3 x 106 | 30 x 106 | 22 | 22 | 45 to 68 |
*Seeding density is given for each culture vessel type as follows: Dishes and Flasks: Cells per vessel; Culture plates: Cells per well. †The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used. |
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