Useful cell culture numbers for various sizes of dishes and flasks

There are various sizes of dishes and flasks used for cell culture. Some useful cell culture numbers such as surface area and volumes of dissociation solutions are given below for various size culture vessels.

 Cat. No.Surface area (cm2)Seeding density*Cells at confluencyVersene
(mL of 0.05% EDTA), approx. volume
Trypsin
(mL of 0.05% trypsin, 0.53 mM EDTA), approx. volume
Growth medium
(mL), approx. volume
Dishes
35 mm150460
150318
8.80.3 x 1061.2 x 106112
60 mm150462 
150288
21.50.8 x 1063.2 x 106335
100 mm150464
150350
56.72.2 x 1068.8 x 1065512
150 mm150468
168381
1455.0 x 10620.0 x 106101030
Culture plates
6-well1406759.60.3 x 1061.2 x 106111 to 3
12-well1506283.50.1 x 1060.5 x 1060.4 to 10.4 to 11 to 2
24-well1424751.90.05 x 1060.24 x 1060.2 to 0.30.2 to 0.30.5 to 1.0
48-well1506871.10.03 x 1060.12 x 1060.1 to 0.20.1 to 0.20.2 to 0.4
96-well1670080.320.01 x 1060.04 x 1060.05 to 0.10.05 to 0.10.1 to 0.2
384-well1646880.0840.0018 x 1060.0072 x 1060.0150.0150.08
1536-well2536140.030.0009 x 1060.0036 x 1060.0030.0030.01
Flasks
T-25156367
156340
250.7 x 1062.8 x 106333 to 5
T-75156499
156472
752.1 x 1068.4 x 106558 to 15
T-175159910
159920
1754.9 x 10623.3 x 106171735 to 53
T-225159934
159933
2256.3 x 10630 x 106222245 to 68
*Seeding density is given for each culture vessel type as follows: Dishes and Flasks: Cells per vessel; Culture plates: Cells per well.

†The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used.


Useful tools for cell culture

Thermo Fisher Scientific offers a wide array of helpful tools and resources to help you find the best products for your work:

Gibco Media Selection Tool

Use the interactive Gibco Media Selection Tool to find a media solution suited to your specific application. Select from catalog media or personalize your formulation to fit your experimental needs and budget.

Cell Culture Select Tool

Use the Cell Culture Select Tool to find resources to help you culture with confidence. Enter your cell line to find recommended, media, sera, and cultureware to support cell growth and maintenance.

Transfection Select Tool

The TransfectionSelect tool can help you find transfection products, protocols, and citations for your research. Enter details about your research and application and receive transfection product recommendations.

Nunc Cell Culture Plastics support every step of your research with a broad range of culture vessels.

Gibco FBS offers consistent growth for your cells through essential nutrients and growth factors.

Resources

Cell Culture & Transfection Learning Center

Access cell culture and transfection educational resources to enable excellent experiment planning and execution.

Gibco Cell Culture Basics

Learn best practices and fundamentals of cell culture. Topics include aseptic technique and methods for the growth and maintenance of cells in culture.

Cell Culture Videos

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Cell Culture Selection Tools

Find the right cell culture products for your research with these selection tools. Search for media, FBS, plastics, transfection reagents, and more.

Support

Cell Culture & Transfection Support Center

Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

Need technical support? 

Contact our expert team for technical and application support of Laboratory Products.

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