Microplate Assays

Explore a broad array of microplate assays by modality, application, and instrument compatibility.

Category	Product	Wavelength	Multiskan FC	Multiskan SkyHigh	Varioskan ALF	Varioskan LUX
Cell viability	alamarBlue Cell Viability Reagent	570 nm	✓	✓	✓	✓
Cell viability	alamarBlue HS Cell Viability Reagent	570 nm	✓	✓	✓	✓
Cell viability	CyQUANT LDH Cytotoxicity	590 nm	✓	✓	✓	✓
Cell viability	CyQUANT MTT Cell viability Assay	570 nm	✓	✓	✓	✓
Cell viability	CyQUANT XTT Cell viability Assay	450 nm and 660 nm	✓	✓	✓	✓
Cell viability	PrestoBlue Cell viability Reagent	570 nm	✓	✓	✓	✓
Cell viability	PrestoBlue HS Cell viability Reagent	570 nm	✓	✓	✓	✓
ELISA	Invitrogen ELISA Kits (Coated or Uncoated) - Choose from >2500 targets	450 nm	✓	✓	✓	✓
Endotoxin quantification	Pierce Chromogenic Endotoxin Quant Kit	405 nm	✓	✓	✓	✓
Peptide Assays	Pierce Dilution-Free Rapid Gold BCA Protein Assay	480 nm	✓	✓	✓	✓
Protein assays	Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards	562 nm	✓	✓	✓	✓
Protein assays	Pierce Bradford Plus Protein Assay Kit with Dilution-Free BSA Protein Standards	595 nm	✓	✓	✓	✓
Protein assays	Micro BCA Protein Assay Kit	562 nm	✓	✓	✓	✓
Protein assays	Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible	562 nm	✓	✓	✓	✓
Protein assays	Pierce BCA Protein Assay Kit - Reducing Agent Compatible	562 nm	✓	✓	✓	✓
Protein assays	Pierce Detergent Compatible Bradford Assay Kit	595 nm	✓	✓	✓	✓
Protein assays	Pierce 660nm Protein Assay Kit	660 nm	✓	✓	✓	✓
Protein assays	Pierce Modiﬁed Lowry Protein Assay Kit	750 nm	✓	✓	✓	✓
Protein assays	Pierce Quantitative Colorimetric Peptide Assay	480 nm	✓	✓	✓	✓

Category	Product	Wavelength	Varioskan ALF	Varioskan LUX
Cell function reagents	ATP Determination Kit	560 nm	✓	✓
Cell viability	QuantiGene Singleplex Gene Expression Assay Kit		✓	✓
Cell viability	Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System		✓	✓
Cell viability	NovaBright Phospha-Light EXP Assay Kit for SEAP Reporter Gene Detection		✓	✓
Cell viability	Galacto-Star β-Galactosidase Reporter Gene Assay System for Mammalian Cells		✓	✓

Category	Product	Wavelength	Varioskan ALF	Varioskan LUX
Peroxidase assay	Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit	571/585 nm	✓	✓
Cell proliferation	Click-iT EdU Proliferation Assay for Microplates	568/585 nm		✓
Cell proliferation	CyQUANT Cell Proliferation	480/520 nm		✓
Cell proliferation	CyQUANT NF (No Freeze) Cell Proliferation	497/520 nm		✓
Cell signaling and lipids	Amplex Red Cholesterol Assay Kit	571/585 nm	✓	✓
Cell signaling and lipids	EnzChek Phosphatase Assay Kit	360/460 nm	✓	✓
Cell viability	alamarBlue Cell viability Reagent	560/590 nm	✓	✓
Cell viability	alamarBlue HS Cell viability Reagent	560/590 nm	✓	✓
Cell viability	CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay)	560/590 nm	✓	✓
Cell viability	CyQUANT LDH Cytotoxicity Assay, fluorescence	560/590 nm	✓	✓
Cell viability	LIVE/DEAD BacLight Bacterial Viability Kit	485/530 nm 630 nm		✓
Cell viability	LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells	494/517 nm 517/617 nm		✓
Cell viability	LIVE/DEAD Yeast Viability Kit	485/530 nm		✓
Cell viability	PrestoBlue Cell viability Reagent	560/590 nm	✓	✓
Cell viability	PrestoBlue HS Cell viability Reagent	560/590 nm	✓	✓
Endotoxin quantification	Quant-iT Endotoxin Detection Assay Kit	490/525 nm	✓	✓
Enzyme activity	EnzChek Protease Assay Kit, green fluorescence	505/513 nm	✓	✓
Enzyme activity	EnzChek Protease Assay Kit, red fluorescence	589/617 nm	✓	✓
pH indicators	pHrodo Green AM Intracellular pH indicator	509/533 nm	✓	✓
pH indicators	pHrodo Red AM Intracellular pH indicator	560/585 nm	✓	✓
Ion indicators	Measure-IT Thiol Assay Kit		✓	✓
Ion indicators	FluoVolt Membrane Potential Kit		✓	✓
Ion indicators	Fluo-4 Direct Calcium Assay Kit		✓	✓
Ion indicators	Fura-2, AM, cell permeant		✓	✓
Ion indicators	FluxOR II Green Potassium Ion Channel Assay		✓	✓
Nucleic acids	Quant-IT dsDNA Assay Kit, high sensitivity (HS) and broad range (BR)	502/523 nm	✓	✓
Nucleic acids	Quant-iT 1X dsDNA Assay Kit, high sensitivity (HS) and broad range (BR)	502/523 nm	✓	✓
Nucleic acids	Quant-iT microRNA Assay Kit	500/525 nm	✓	✓
Nucleic acids	Quant-iT OliGreen ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent	500/525 nm	✓	✓
Nucleic acids	Quant-iT RNA Assay Kit and Quant-iT RNA HS Reagent	644/673 nm	✓	✓
Peptide assays	Pierce Quantitative Fluorometric Peptide Assay	390/475 nm	✓	✓
Protein assays	CBQCA Plus Protein Quantitation Kit	465/550 nm	✓	✓
Protein assays	NanoOrange Protein Quantitation Kit	485/590 nm	✓	✓
Protein assays	Quant-iT Protein Assay Kit	485/590 nm	✓	✓
Protein assays	EZQ Protein Quantitation Kit	485/590 nm	✓	✓
Reactive oxygen species	CellROX Deep Red Reagent, for oxidative stress detection	644/665 nm	✓	✓
Reactive oxygen species	CellROX Green Reagent, for oxidative stress detection	485/520 nm	✓	✓