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Quant-iT Technology
Invitrogen Quant-iT assay kits use intentionally designed fluorescent dyes which bind selectively to DNA, RNA, protein, or endotoxin. The intensity of the fluorescent signal of the resulting complex is proportional to the amount of the target molecule in the sample.
Key benefits of Quant-iT assays:
- Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
- Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold, giving a very high signal-to-background ratio resulting in accurate low-concentration measurements—up to 1,000 times more sensitive than absorbance-based readings.
- Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you can get the crucial quantitation data you need, without wasting your sample.
Which assay kit is right for me?
| 1–20 samples | 20–2,000 samples | > 2,000 samples |
|---|---|---|
| Qubit assay kits for use with the Qubit Fluorometers | Quant-iT assay kits and reagents for use with microplate readers* | Quant-iT bulk reagents and kits |
Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples
Product | Target | Useful range† | Ex/Em‡ | No. of assays | Cat. No. |
|---|---|---|---|---|---|
| Quant-iT PicoGreen ReadyPlate | dsDNA | 0.2–200 ng | 502/523 | 96 | P32705 |
| Quant-iT 1X dsDNA HS Assay | dsDNA | 0.2–100 ng | 510/527 | 1,000 | Q33232 |
| Quant-iT dsDNA High-Sensitivity (HS) Assay Kit | dsDNA | 0.2–100 ng | 510/527 | 1,000 | Q33120 |
| Quant-iT 1X dsDNA BR Assay | dsDNA | 4-4,000 ng | 510/527 | 1,000 | Q33267 |
| Quant-iT dsDNA Broad-Range (BR) Assay Kit | dsDNA | 4–1,000 ng | 510/527 | 1,000 | Q33130 |
| Quant-iT RiboGreen ReadyPlate | RNA | 1–200 ng | 500/525 | 96 | R32705 |
| Quant-iT RNA Assay Kit Quant-iT RNA Assay Reagent | RNA | 5–100 ng | 644/673 | 1,000 | Q33140 Q32884 |
| Quant-iT RNA Broad-Range BR Assay Kit | RNA | 20–1,000 ng | 644/673 | 1,000 | Q10213 |
| Quant-iT RNA XR Assay Kit | RNA | 200–10,000 ng | 644/673 | 1,000 | Q33225 |
| Quant-iT microRNA Assay Kit | microRNA | 1–100 ng | 500/525 | 1,000 | Q32882 |
| Quant-iT Protein Assay Kit | Protein | 0.25–5 µg | 470/570 | 1,000 | Q33210 |
| Quant-iT Endotoxin Detection Assay Kit | Endotoxin | 0.01–10.0 EU/mL | 490/525 | 160 | Q32892 |
*Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.
Quant-iT bulk reagents and kits for >2,000 samples
Product | Target | Useful range† | Ex/Em‡ | No. of assays | Cat. No. |
|---|---|---|---|---|---|
| Quant-iT PicoGreen dsDNA Assay Kit (1 mL) Quant-iT PicoGreen dsDNA Reagent (1 mL) | dsDNA | 50 pg–2 µg | 502/523 | 2,000 | P7589 P7581 |
| Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) | dsDNA | 50 pg–2 µg | 502/523 | 2,000 | P11496 P11495 |
| Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent | ssDNA | 200 pg–2 µg | 500/525 | 2,000 | O11492 O7582 |
| Quant-iT RiboGreen RNA Assay Kit Quant-iT RiboGreen RNA Reagent | RNA | 1–200 ng | 500/525 | 2,000 | R11490 R11491 |
| RediPlate 96 RiboGreen RNA Quantitation Kit | RNA | 3–200 ng | 500/525 | 96 | R32700 |
* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent.
Quant-iT assay accessories±
± Validated for Quant-iT Assays.
Expertly detect fluorescence with Thermo Scientific plate readers
High-sensitivity fluorescence detection of nucleic acids or protein for 6–1,536 samples can be quickly performed on the Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for accurate and precise detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Easy choices for RNA and DNA quantification
Choosing the right technology for your samples and workflow can save significant time and money by helping to prevent downstream experimental failures.
Resources
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