Organism TypeHamsterThe CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth. ATCC CCL-61
TissuesOvary
PhenotypeAdherent
Primaryno
ApplicationCell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection

Recommended Transfection Reagents

  • Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
  • RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Catalog # Name Size Price (USD) Qty
10131027 Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL), 100 mL Each
700.00
10131035 Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL), 20 mL Each
179.65

Online exclusive

197.00
Save 17.35 (9%)
10313021 DMEM, high glucose, pyruvate, no glutamine, 500 mL Each
34.65

Online exclusive

39.83
Save 5.18 (13%)
10459014 DMRIE-C Transfection Reagent Each
724.00
10687010 Hygromycin B (50 mg/mL) Each
278.00
10964013 10964013 -
11140050 MEM Non-Essential Amino Acids Solution (100X), 100 mL Each
25.65

Online exclusive

28.42
Save 2.77 (10%)
11140076 MEM Non-Essential Amino Acids Solution (100X), 20 x 100 mL Each
463.65

Online exclusive

514.00
Save 50.35 (10%)
11514015 PLUS™ Reagent 0.85 mL
380.00
11668019 Lipofectamine™ 2000 Transfection Reagent, 1.5 mL Each
804.65

Online exclusive

882.00
Save 77.35 (9%)
11668027 Lipofectamine™ 2000 Transfection Reagent, 0.75 mL Each
519.65

Online exclusive

562.00
Save 42.35 (8%)
11765047 Ham's F-12 Nutrient Mix, 1000 mL Each
56.65

Online exclusive

61.75
Save 5.10 (8%)
11765054 Ham's F-12 Nutrient Mix, 500 mL 500 mL
36.65

Online exclusive

39.74
Save 3.09 (8%)
11765062 Ham's F-12 Nutrient Mix, 10 x 500 mL Each
312.65

Online exclusive

343.00
Save 30.35 (9%)
11765070 Ham's F-12 Nutrient Mix, 6 x 1000 mL Each
305.65

Online exclusive

331.00
Save 25.35 (8%)
11811023 Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 1 g Each
167.65

Online exclusive

182.00
Save 14.35 (8%)
11811031 Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 5 g Each
493.65

Online exclusive

536.00
Save 42.35 (8%)
11811098 Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 25 g Each
2,076.65

Online exclusive

2,255.00
Save 178.35 (8%)
11960044 DMEM, high glucose, no glutamine, 500 mL Each
32.65

Online exclusive

35.81
Save 3.16 (9%)
11960051 DMEM, high glucose, no glutamine, 1000 mL Each
47.65

Online exclusive

56.75
Save 9.10 (16%)
11960069 DMEM, high glucose, no glutamine, 10 x 500 mL Each
282.65

Online exclusive

308.00
Save 25.35 (8%)
11960077 DMEM, high glucose, no glutamine, 6 x 1000 mL Each
271.65

Online exclusive

296.00
Save 24.35 (8%)
11965084 DMEM, high glucose, 1000 mL Each
52.65

Online exclusive

57.75
Save 5.10 (9%)
11965092 DMEM, high glucose, 500 mL Each
32.65

Online exclusive

35.34
Save 2.69 (8%)
11965118 DMEM, high glucose, 10 x 500 mL Each
276.65

Online exclusive

304.00
Save 27.35 (9%)
11965126 DMEM, high glucose, 6 x 1000 mL Each
266.65

Online exclusive

293.00
Save 26.35 (9%)
12052114 CHO-S-SFM II, with hypoxanthine and thymidine, 500 mL Each
100.00
12571048 MEM α, nucleosides, 1000 mL Each
87.65

Online exclusive

98.50
Save 10.85 (11%)
12571063 MEM α, nucleosides, 500 mL Each
56.65

Online exclusive

61.75
Save 5.10 (8%)
12571071 MEM α, nucleosides, 10 x 500 mL Each
488.65

Online exclusive

538.00
Save 49.35 (9%)
15070063 Penicillin-Streptomycin (5,000 U/mL) Each
25.65

Online exclusive

27.36
Save 1.71 (6%)
15140122 Penicillin-Streptomycin (10,000 U/mL), 100 mL Each
31.45
15140148 Penicillin-Streptomycin (10,000 U/mL), 20 mL Each
16.62
15338100 Lipofectamine™ LTX Reagent with PLUS™ Reagent, 1 mL Each
814.65

Online exclusive

896.00
Save 81.35 (9%)
15338500 Lipofectamine™ LTX Reagent Each
12,080.00
15640055 Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture Each
48.65

Online exclusive

51.00
Save 2.35 (5%)
16000036 Fetal Bovine Serum, certified, United States 100 mL
231.00
16000044 Fetal Bovine Serum, certified, United States, 500 mL Each -
16000069 Fetal Bovine Serum, certified, United States, 1000 mL Each -
18292011 Lipofectin™ Transfection Reagent, 1 mL Each
678.00
18324012 Lipofectamine™ Transfection Reagent, 1 mL Each
650.00
18324020 Lipofectamine™ Transfection Reagent, 4 mL Each
2,200.00
18324111 Lipofectamine™ Transfection Reagent -"DISCONTINUED" 0.5 mL -
21127022 Ham's F-12K (Kaighn's) Medium, 500 mL Each
39.97
26400036 Fetal Bovine Serum, dialyzed , US origin 100 mL
282.00
26400044 Fetal Bovine Serum, dialyzed, US origin, 500 mL Each
1,142.00
31985062 Opti-MEM™ I Reduced Serum Medium, 100 mL Each
29.65

Online exclusive

32.67
Save 3.02 (9%)
K278001 Calcium Phosphate Transfection Kit Each
700.00
R21001 Blasticidin S HCl, powder Each
286.00
R25001 Zeocin™ Selection Reagent, 8 x 1.25 mL Each
327.65

Online exclusive

362.00
Save 34.35 (9%)
V04450 pCEP4 Mammalian Expression Vector 20 µg
990.00
V22020 pcDNA™6/V5-His A, B, & C Mammalian Expression Vectors 20 µg -
V48401 pVP22/myc-His Vector Kit -"DISCONTINUED" 20 µg -
V79020 pcDNA™3.1 (+) Mammalian Expression Vector 20 µg
1,062.00
V79520 pcDNA™3.1 (-) Mammalian Expression Vector 20 µg
1,040.00
V87120 pTracer™-SV40 Mammalian Expression Vector 20 µg -
V88320 pTracer™-CMV/Bsd Mammalian Expression Vector 20 µg -
V88501 pTracer™-CMV2 Kit 1 kit -
V88520 pTracer™-CMV2 Mammalian Expression Vector 20 µg -
V88720 pTracer™-EF A, B, & C Mammalian Expression Vectors 20 µg -
V90020 pSecTag2 A, B, & C Mammalian Expression Vectors 20 µg
1,288.00

Every step of the way, a wide range of cell health products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.