cho k1 Cells | Thermo Fisher Scientific

Organism Type	Hamster	The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth. ATCC CCL-61
Tissues	Ovary
Phenotype	Adherent
Primary	no
Application	Cell Culture / Growth Conditions,Protein Expression,Stable Cell Transfection,Transfection: Gene Expression,Transient Transfection

Recommended Transfection Reagents

Plasmid DNA transfection and co-transfection: Lipofectamine® 3000 (51-79% transfection efficiency and 1 fold protein expression improvement over Lipofectamine® 2000)
RNAi transfection (siRNA/miRNA) and gene silencing: Lipofectamine® RNAiMAX

Products

Catalog #	Name	Size	Price (USD)	Qty
10131027	Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL), 100 mL	Each	736.00
10131035	Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL), 20 mL	Each	188.65 Online Exclusive ~~207.00~~ Save 18.35 (9%)
10313021	DMEM, high glucose, pyruvate, no glutamine, 500 mL	Each	36.65 Online Exclusive ~~41.86~~ Save 5.21 (12%)
10459014	DMRIE-C Transfection Reagent	Each	760.00
10687010	Hygromycin B (50 mg/mL)	Each	292.00
10964013	10964013		Contact Us ›	-
11140050	MEM Non-Essential Amino Acids Solution (100X), 100 mL	Each	26.65 Online Exclusive ~~29.87~~ Save 3.22 (11%)
11140076	MEM Non-Essential Amino Acids Solution (100X), 20 x 100 mL	Each	487.65 Online Exclusive ~~540.00~~ Save 52.35 (10%)
11514015	PLUS™ Reagent	0.85 mL	398.00
11668019	Lipofectamine™ 2000 Transfection Reagent, 1.5 mL	Each	842.65 Online Exclusive ~~924.00~~ Save 81.35 (9%)
11668027	Lipofectamine™ 2000 Transfection Reagent, 0.75 mL	Each	545.65 Online Exclusive ~~590.00~~ Save 44.35 (8%)
11765047	Ham's F-12 Nutrient Mix, 1000 mL	Each	59.65 Online Exclusive ~~65.00~~ Save 5.35 (8%)
11765054	Ham's F-12 Nutrient Mix, 500 mL	500 mL	38.65 Online Exclusive ~~41.77~~ Save 3.12 (7%)
11765062	Ham's F-12 Nutrient Mix, 10 x 500 mL	Each	328.65 Online Exclusive ~~360.00~~ Save 31.35 (9%)
11765070	Ham's F-12 Nutrient Mix, 6 x 1000 mL	Each	321.65 Online Exclusive ~~348.00~~ Save 26.35 (8%)
11811023	Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 1 g	Each	175.65 Online Exclusive ~~191.00~~ Save 15.35 (8%)
11811031	Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 5 g	Each	519.65 Online Exclusive ~~564.00~~ Save 44.35 (8%)
11811098	Geneticin™ Selective Antibiotic (G418 Sulfate), Powder, 25 g	Each	2,182.65 Online Exclusive ~~2,370.00~~ Save 187.35 (8%)
11960044	DMEM, high glucose, no glutamine, 500 mL	Each	34.65 Online Exclusive ~~37.64~~ Save 2.99 (8%)
11960051	DMEM, high glucose, no glutamine, 1000 mL	Each	50.65 Online Exclusive ~~59.75~~ Save 9.10 (15%)
11960069	DMEM, high glucose, no glutamine, 10 x 500 mL	Each	297.65 Online Exclusive ~~324.00~~ Save 26.35 (8%)
11960077	DMEM, high glucose, no glutamine, 6 x 1000 mL	Each	285.65 Online Exclusive ~~311.00~~ Save 25.35 (8%)
11965084	DMEM, high glucose, 1000 mL	Each	55.65 Online Exclusive ~~60.75~~ Save 5.10 (8%)
11965092	DMEM, high glucose, 500 mL	Each	34.65 Online Exclusive ~~37.14~~ Save 2.49 (7%)
11965118	DMEM, high glucose, 10 x 500 mL	Each	291.65 Online Exclusive ~~320.00~~ Save 28.35 (9%)
11965126	DMEM, high glucose, 6 x 1000 mL	Each	280.65 Online Exclusive ~~308.00~~ Save 27.35 (9%)
12052114	CHO-S-SFM II, with hypoxanthine and thymidine, 500 mL	Each	100.00
12571048	MEM α, nucleosides, 1000 mL	Each	92.65 Online Exclusive ~~104.00~~ Save 11.35 (11%)
12571063	MEM α, nucleosides, 500 mL	Each	59.65 Online Exclusive ~~65.00~~ Save 5.35 (8%)
12571071	MEM α, nucleosides, 10 x 500 mL	Each	513.65 Online Exclusive ~~566.00~~ Save 52.35 (9%)
15070063	Penicillin-Streptomycin (5,000 U/mL)	Each	26.65 Online Exclusive ~~28.76~~ Save 2.11 (7%)
15140122	Penicillin-Streptomycin (10,000 U/mL), 100 mL	Each	33.05
15140148	Penicillin-Streptomycin (10,000 U/mL), 20 mL	Each	17.47
15338100	Lipofectamine™ LTX Reagent with PLUS™ Reagent, 1 mL	Each	854.65 Online Exclusive ~~940.00~~ Save 85.35 (9%)
15338500	Lipofectamine™ LTX Reagent	Each	12,670.00
15640055	Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture	Each	51.65 Online Exclusive ~~53.50~~ Save 1.85 (3%)
16000044	Fetal Bovine Serum, certified, United States, 500 mL	Each	Request A Quote	-
16000069	Fetal Bovine Serum, certified, United States, 1000 mL	Each	Request A Quote	-
18292011	Lipofectin™ Transfection Reagent, 1 mL	Each	710.00
18324012	Lipofectamine™ Transfection Reagent, 1 mL	Each	682.00
18324020	Lipofectamine™ Transfection Reagent, 4 mL	Each	2,305.00
21127022	Ham's F-12K (Kaighn's) Medium, 500 mL	Each	42.01
26400044	Fetal Bovine Serum, dialyzed, US origin, 500 mL	Each	1,182.00
31985062	Opti-MEM™ I Reduced Serum Medium, 100 mL	Each	31.65 Online Exclusive ~~34.34~~ Save 2.69 (8%)
K278001	Calcium Phosphate Transfection Kit	Each	734.00
R21001	Blasticidin S HCl, powder	Each	301.00
R25001	Zeocin™ Selection Reagent, 8 x 1.25 mL	Each	343.65 Online Exclusive ~~380.00~~ Save 36.35 (10%)
V04450	pCEP4 Mammalian Expression Vector	20 µg	1,034.00
V79020	pcDNA™3.1⁽⁺⁾ Mammalian Expression Vector	20 µg	1,110.00
V79520	pcDNA™3.1^(-) Mammalian Expression Vector	20 µg	1,086.00
V90020	pSecTag2 A, B, & C Mammalian Expression Vectors	20 µg	1,346.00

Every step of the way, a wide range of cell health products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.

GROW Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter		MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology		DETECT Invitrogen imaging reagents Invitrogen flow cytometry reagents		ANALYZE EVOS cell imaging systems Attune NxT Flow Cytometer Qubit fluorometric quantification